• Immunohistochemistry analysis of CGN in paraffin-embedded Human colon carcinoma using CGN Rabbit polyclonal antibody (STJ117684) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using CGN Rabbit polyclonal antibody (STJ117684) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CGN antibody (904-1203) (STJ117684)

SKU:
STJ117684

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CGN (904-1203) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CGN
Gene ID: 57530
Uniprot ID: CING_HUMAN
Immunogen Region: 904-1203
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 904-1203 of human CGN (NP_065821.1).
Immunogen Sequence: TSGGLSRLQDEIQRLRQALQ ASQAERDTARLDKELLAQRL QGLEQEAENKKRSQDDRARQ LKGLEEKVSRLETELDEEKN TVELLTDRVNRGRDQVDQLR TELMQERSARQDLECDKISL ERQNKDLKTRLASSEGFQKP SASLSQLESQNQLLQERLQA EEREKTVLQSTNRKLERKVK ELSIQIEDERQHVNDQKDQL SLRVKALKRQVDEAEEEIER LDGLRKKAQREVEEQHEVN
Tissue Specificity Localized on the cytoplasmic face of tight junctions of polarized epithelia and some endothelia. Expressed in pancreas, kidney, liver and lung, but not in skeletal muscle, placenta, brain or heart.
Function Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.
Protein Name Cingulin
Database Links Reactome: R-HSA-2173791
Cellular Localisation Cell Junction
Tight Junction
Localizes To The Apical Junction Complex Composed Of Tight And Adherens Junctions
Colocalizes With Spef1 At Sites Of Cell-Cell Contact In Intestinal Epithelial Cells
Alternative Antibody Names Anti-Cingulin antibody
Anti-CGN antibody
Anti-KIAA1319 antibody

Information sourced from Uniprot.org

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