• Western blot analysis of extracts of various cell lines, using CFL2 antibody (STJ110316) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of A549 cells using CFL2 antibody (STJ110316). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of A549 cells using CFL2 antibody (STJ110316). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using CFL2 antibody (STJ110316) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CFL2 antibody (1-166) (STJ110316)

SKU:
STJ110316

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CFL2 (1-166) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CFL2
Gene ID: 1073
Uniprot ID: COF2_HUMAN
Immunogen Region: 1-166
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-166 of human CFL2 (NP_068733.1).
Immunogen Sequence: MASGVTVNDEVIKVFNDMKV RKSSTQEEIKKRKKAVLFCL SDDKRQIIVEEAKQILVGDI GDTVEDPYTSFVKLLPLNDC RYALYDATYETKESKKEDLV FIFWAPESAPLKSKMIYASS KDAIKKKFTGIKHEWQVNGL DDIKDRSTLGEKLGGNVVVS LEGKPL
Tissue Specificity Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues.
Post Translational Modifications The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal.
Function Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. Its F-actin depolymerization activity is regulated by association with CSPR3. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Required for muscle maintenance. May play a role during the exchange of alpha-actin forms during the early postnatal remodeling of the sarcomere.
Protein Name Cofilin-2
Cofilin - Muscle Isoform
Cellular Localisation Nucleus Matrix
Cytoplasm
Cytoskeleton
Colocalizes With Cspr3 In The Z Line Of Sarcomeres
Alternative Antibody Names Anti-Cofilin-2 antibody
Anti-Cofilin - Muscle Isoform antibody
Anti-CFL2 antibody

Information sourced from Uniprot.org

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