• Immunofluorescence analysis of paraffin-embedded mouse pancreas using CELA2A Rabbit polyclonal antibody (STJ11102876) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Mouse lung, using CELA2A Rabbit polyclonal antibody (STJ11102876) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CELA2A antibody (80-150) (STJ11102876)

SKU:
STJ11102876

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CELA2A (80-150) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CELA2A
Gene ID: 63036
Uniprot ID: CEL2A_HUMAN
Immunogen Region: 80-150
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 80-150 of human CELA2A (NP_254275.1).
Immunogen Sequence: TYRVGLGRHNLYVAESGSLA VSVSKIVVHKDWNSNQISKG NDIALLKLANPVSLTDKIQL ACLPPAGTILP
Tissue Specificity Expressed in pancreas. Not detected in keratinocytes. Detected in exocrine secretions of the pancreas (at protein level). Also expressed in a small fraction of cells in pancreatic islets, adrenal cortex, intestinal glands and colonic lymphoid follicles (at protein level). Detected in plasma.
Function Elastase that enhances insulin signaling and might have a physiologic role in cellular glucose metabolism. Circulates in plasma and reduces platelet hyperactivation, triggers both insulin secretion and degradation, and increases insulin sensitivity.
Protein Name Chymotrypsin-Like Elastase Family Member 2a
Elastase-2a
Database Links Reactome: R-HSA-6809371
Cellular Localisation Secreted
Alternative Antibody Names Anti-Chymotrypsin-Like Elastase Family Member 2a antibody
Anti-Elastase-2a antibody
Anti-CELA2A antibody
Anti-ELA2A antibody

Information sourced from Uniprot.org

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