Western blot analysis of extracts of various cell lines, using CEACAM6 antibody (STJ110738) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CEACAM6 antibody (35-344) (STJ110738)

SKU:
STJ110738
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Covered by St John's Laboratory Guarantee

Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:5000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CEACAM6
Gene ID: 4680
Uniprot ID: CEAM6_HUMAN
Immunogen Region: 35-344
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 35-320 of human CEACAM6 (NP_002474.4).
Immunogen Sequence: KLTIESTPFNVAEGKEVLLL AHNLPQNRIGYSWYKGERVD GNSLIVGYVIGTQQATPGPA YSGRETIYPNASLLIQNVTQ NDTGFYTLQVIKSDLVNEEA TGQFHVYPELPKPSISSNNS NPVEDKDAVAFTCEPEVQNT TYLWWVNGQSLPVSPRLQLS NGNMTLTLLSVKRNDAGSYE CEIQNPASANRSDPVTLNVL YGPDVPTISPSKANYRPGEN LNLSCHAASNPPAQYSWFI
Tissue Specificity Expressed in neutrophils. Expressed in columnar epithelial and goblet cells of the colon. Expressed in numerous tumor cell lines (at protein level).
Post Translational Modifications Glycosylated.
Function Cell surface glycoprotein that plays a role in cell adhesion and tumor progression. Intercellular adhesion occurs in a calcium- and fibronectin-independent manner. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8. Heterophilic interaction with CEACAM8 occurs in activated neutrophils. Plays a role in neutrophil adhesion to cytokine-activated endothelial cells. Plays a role in cell migration and cell adhesion to endothelial cells.
Protein Name Cell Adhesion Molecule Ceacam6
Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6
Cea Cell Adhesion Molecule 6
Non-Specific Crossreacting Antigen
Normal Cross-Reacting Antigen
Cd Antigen Cd66c
Database Links Reactome: R-HSA-1566977
Reactome: R-HSA-202733
Reactome: R-HSA-6798695
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Apical Cell Membrane
Cell Surface
Localized To The Apical Glycocalyx Surface
Alternative Antibody Names Anti-Cell Adhesion Molecule Ceacam6 antibody
Anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 antibody
Anti-Cea Cell Adhesion Molecule 6 antibody
Anti-Non-Specific Crossreacting Antigen antibody
Anti-Normal Cross-Reacting Antigen antibody
Anti-Cd Antigen Cd66c antibody
Anti-CEACAM6 antibody
Anti-NCA antibody

Information sourced from Uniprot.org

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