![Western blot analysis of lysates from HepG2 cells, using [KO Validated] CDKN2C/p18-INK4C Rabbit mAb (STJ11102368) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/91285/405985/STJ11102368_2__72200.1744891775.jpg?c=1 "Western blot analysis of lysates from HepG2 cells, using [KO Validated] CDKN2C/p18-INK4C Rabbit mAb (STJ11102368) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.")
![Western blot analysis of lysates from wild type (WT) and CDKN2C knockout (KO) 293T cells, using [KO Validated] CDKN2C/p18-INK4C Rabbit mAb (STJ11102368) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/91285/405986/STJ11102368_3__57041.1744891776.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CDKN2C knockout (KO) 293T cells, using [KO Validated] CDKN2C/p18-INK4C Rabbit mAb (STJ11102368) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.")
Anti-CDKN2C antibody (1-120) [S8MR] (STJ11102368)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal CDKN2C/p18-INK4C (1-120) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S8MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CDKN2C |
| Gene ID | 1031 |
| Uniprot ID | CDN2C_HUMAN |
| Immunogen Region | 1-120 |
| Immunogen Sequence | MAEPWGNELASAAARGDLEQ LTSLLQNNVNVNAQNGFGRT ALQVMKLGNPEIARRLLLRG ANPDLKDRTGFAVIHDAARA GFLDTLQTLLEFQADVNIED NEGNLPLHLAAKEGHLRVVE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human CDKN2C/p18-INK4C (P42773). |
Additional Info
| Tissue Specificity | Highest levels found in skeletal muscle. Also found in pancreas and heart. |
| Function | Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB. |
| Protein Name | Cyclin-Dependent Kinase 4 Inhibitor CCyclin-Dependent Kinase 6 InhibitorP18-Ink4cP18-Ink6 |
| Database Links | Reactome: R-HSA-2559580Reactome: R-HSA-2559582Reactome: R-HSA-2559585Reactome: R-HSA-69231 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Cyclin-Dependent Kinase 4 Inhibitor C antibodyAnti-Cyclin-Dependent Kinase 6 Inhibitor antibodyAnti-P18-Ink4c antibodyAnti-P18-Ink6 antibodyAnti-CDKN2C antibodyAnti-CDKN6 antibody |
Information sourced from Uniprot.org