• Western blot detection of CDK4 in Hela, C6 and F9 cell lysates using CDK4 mouse mAb (1:1000 diluted).Predicted band size:34KDa.Observed band size:34KDa.
  • Western blot detection of CDK4 in Hela, Lncap and F9 cell lysates using CDK4 mouse mAb (1:1000 diluted).Predicted band size:34KDa.Observed band size:33KDa.

Anti-CDK4 antibody (STJ99110)

SKU:
STJ99110
Current Stock:
Host: Mouse
Applications: WB
Reactivity: Human,Mouse,Rat
Short Description: Mouse monoclonal primary antibody against CDK4.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Monoclonal
Clone ID: 3F9-B12-C8
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG1
Dilution Range: WB 1:1000
Specificity: This antibody detects endogenous levels of CDK4 and does not cross-react with related proteins.
Uniprot ID: CDK4_HUMAN
Gene ID: 1019
Gene Symbol: CDK4
Immunogen: Purified recombinant human CDK4 protein fragments expressed in E.coli.
Post Translational Modifications Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.
Function Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Database Links Reactome: R-HSA-187577
Cellular Localisation Cytoplasm
Nucleus
Nucleus Membrane
Cytoplasmic When Non-Complexed
Forms A Cyclin D-Cdk4 Complex In The Cytoplasm As Cells Progress Through G(1) Phase
The Complex Accumulates On The Nuclear Membrane And Enters The Nucleus On Transition From G(1) To S Phase
Also Present In Nucleoli And Heterochromatin Lumps
Colocalizes With Rb1 After Release Into The Nucleus
Protein Name Cyclin-Dependent Kinase 4
Cell Division Protein Kinase 4
Psk-J3
Alternative Names Anti-Cyclin-Dependent Kinase 4 antibody
Anti-Cell Division Protein Kinase 4 antibody
Anti-Psk-J3 antibody
Anti-CDK4 antibody
6 months standard