• Immunofluorescence analysis of NIH-3T3 cells using CDK9 Rabbit mAb (STJ11101279) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug CDK9 antibody (STJ11101279). Western blot was performed from the immunoprecipitate using CDK9 antibody (STJ11101279) at a dilition of 1:1000.
  • Immunofluorescence analysis of HeLa cells using CDK9 Rabbit mAb (STJ11101279) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded mouse brain using CDK9 Rabbit mAb (STJ11101279) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human esophageal using CDK9 Rabbit mAb (STJ11101279) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded rat ovary using CDK9 Rabbit mAb (STJ11101279) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using CDK9 Rabbit mAb (STJ11101279) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3min.

Anti-CDK4 antibody (STJ11101279)

SKU:
STJ11101279
Current Stock:
Host: Rabbit
Applications: WB, IF
Reactivity: Human
Short Description: Rabbit monoclonal primary antibody against CDK4.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Monoclonal
Clone ID: ARC0526
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IF: 1:50-1:200
Uniprot ID: CDK4_HUMAN
Gene ID: 1019
Gene Symbol: CDK4
Immunogen: A synthesized peptide derived from human CDK4
Post Translational Modifications Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.
Function Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Database Links Reactome: R-HSA-187577
Cellular Localisation Cytoplasm
Nucleus
Nucleus Membrane
Cytoplasmic When Non-Complexed
Forms A Cyclin D-Cdk4 Complex In The Cytoplasm As Cells Progress Through G(1) Phase
The Complex Accumulates On The Nuclear Membrane And Enters The Nucleus On Transition From G(1) To S Phase
Also Present In Nucleoli And Heterochromatin Lumps
Colocalizes With Rb1 After Release Into The Nucleus
Protein Name Cyclin-Dependent Kinase 4
Cell Division Protein Kinase 4
Psk-J3
Alternative Names Anti-Cyclin-Dependent Kinase 4 antibody
Anti-Cell Division Protein Kinase 4 antibody
Anti-Psk-J3 antibody
Anti-CDK4 antibody
6 months standard