• Various whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-N-Cadherin (ABT-CDH2) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HepG2 Lane 2: Hela Lane 3: A431Predicted band size: 100kDaObserved band size: 110kDa
  • Human Kidney tissue was stained with Anti-N-Cadherin (ABT-CDH2) Antibody
  • Human liver tissue was stained with Anti-N-Cadherin (ABT-CDH2) Antibody
  • Human liver tissue was stained with Anti-N-Cadherin (ABT-CDH2) Antibody
  • Immunohistochemical analysis of paraffin-embedded Liver. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Liver. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Western blot analysis of N-CadherinAntibody at 1:1000 dilution.
  • Various whole cell lysates were separated by 15% SDS-PAGE, and the membrane was blotted with anti-N-Cadherin (ABT-CDH2) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: C2C12 Lane 2: HEK293 Lane 2: HepG2Predicted band size: 100kDaObserved band size: 110kDa

Anti-CDH2 antibody (200-400 aa) [ABT-CDH2] (STJ196931)

SKU:
STJ196931

$226.80 - $305.10
Current Stock:
Host: Mouse
Applications: WB/IHC/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Cadherin-2 (200-400 aa) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: ABT-CDH2
Conjugation: Unconjugated
Isotype: IgG2bk
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: IHC-P 1:100-500
WB 1:500-2000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDH2
Gene ID: 1000
Uniprot ID: CADH2_HUMAN
Immunogen Region: 200-400 aa
Specificity: This antibody detects endogenous levels of human N-Cadherin. Heat-induced epitope retrieval (HIER) Citrate buffer of pH6.0 was highly recommended as antigen repair method in paraffin section
Immunogen: Synthesized peptide derived from the human N-Cadherin at the amino acid range 200-400
Function Calcium-dependent cell adhesion protein.preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells. Required for proper neurite branching. Required for pre- and postsynaptic organization. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
Protein Name Cadherin-2
Cdw325
Neural Cadherin
N-Cadherin
Cd Antigen Cd325
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-418990
Reactome: R-HSA-525793
Reactome: R-HSA-8957275
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Sarcolemma
Cell Junction
Cell Surface
Desmosome
Adherens Junction
Colocalizes With Tmem65 At The Intercalated Disk In Cardiomyocytes
Colocalizes With Obscn At The Intercalated Disk And At Sarcolemma In Cardiomyocytes
Alternative Antibody Names Anti-Cadherin-2 antibody
Anti-Cdw325 antibody
Anti-Neural Cadherin antibody
Anti-N-Cadherin antibody
Anti-Cd Antigen Cd325 antibody
Anti-CDH2 antibody
Anti-CDHN antibody
Anti-NCAD antibody

Information sourced from Uniprot.org

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