• Immunohistochemistry analysis of paraffin-embedded mouse heart using CDC42EP1 Rabbit monoclonal antibody (STJ11103098) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using CDC42EP1 Rabbit monoclonal antibody (STJ11103098) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CDC42EP1 Rabbit monoclonal antibody (STJ11103098) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CDC42EP1 antibody (292-391) [S8MR] (STJ11103098)

SKU:
STJ11103098

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CDC42EP1 (292-391) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDC42EP1
Gene ID: 11135
Uniprot ID: BORG5_HUMAN
Immunogen Region: 292-391
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 292-391 of human CDC42EP1 (Q00587).
Immunogen Sequence: AGLGPVAEVKSSPVGGGPRG PAGPALGRHWGAGWDGGHHY PEMDARQERVEVLPQARASW ESLDEEWRAPQAGSRTPVPS TVQANTFEFADAEEDDEVKV
Tissue Specificity Endothelial and bone marrow stromal cells.
Function Probably involved in the organization of the actin cytoskeleton. Induced membrane extensions in fibroblasts.
Protein Name Cdc42 Effector Protein 1
Binder Of Rho Gtpases 5
Serum Protein Mse55
Database Links Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013406
Reactome: R-HSA-9013408
Reactome: R-HSA-9013409
Reactome: R-HSA-9013423
Cellular Localisation Endomembrane System
Peripheral Membrane Protein
Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Cdc42 Effector Protein 1 antibody
Anti-Binder Of Rho Gtpases 5 antibody
Anti-Serum Protein Mse55 antibody
Anti-CDC42EP1 antibody
Anti-BORG5 antibody
Anti-CEP1 antibody
Anti-MSE55 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance