• Immunofluorescence analysis of HeLa cells using CDC25A Rabbit polyclonal antibody (STJ23024) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using CDC25A antibody (STJ23024) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver damage using CDC25A antibody (STJ23024) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat liver using CDC25A antibody (STJ23024) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CDC25A antibody (STJ23024) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CDC25A antibody (86-328) (STJ23024)

SKU:
STJ23024

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CDC25A (86-328) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDC25A
Gene ID: 993
Uniprot ID: MPIP1_HUMAN
Immunogen Region: 86-328
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 86-328 of CDC25A (NP_001780.2).
Immunogen Sequence: LDSPGPLDSKENLENPMRRI HSLPQKLLGCSPALKRSHSD SLDHDIFQLIDPDENKENEA FEFKKPVRPVSRGCLHSHGL QEGKDLFTQRQNSAPARMLS SNERDSSEPGNFIPLFTPQS PVTATLSDEDDGFVDLLDGE NLKNEEETPSCMASLWTAPL VMRTTNLDNRCKLFDSPSLC SSSTRSVLKRPERSQEESPP GSTKRRKSMSGASPKESTNP EKAHETLHQSLSLASSPKG
Post Translational Modifications Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation. Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.
Function Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
Protein Name M-Phase Inducer Phosphatase 1
Dual Specificity Phosphatase Cdc25a
Database Links Reactome: R-HSA-1362277
Reactome: R-HSA-156711
Reactome: R-HSA-176187
Reactome: R-HSA-5689880
Reactome: R-HSA-69202
Reactome: R-HSA-69273
Reactome: R-HSA-69601
Reactome: R-HSA-69656
Reactome: R-HSA-8862803
Alternative Antibody Names Anti-M-Phase Inducer Phosphatase 1 antibody
Anti-Dual Specificity Phosphatase Cdc25a antibody
Anti-CDC25A antibody

Information sourced from Uniprot.org

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