| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Cell division cycle protein 20 homolog (81-130 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:20000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CDC20 |
| Gene ID: | 991 |
| Uniprot ID: | CDC20_HUMAN |
| Immunogen Region: | 81-130 aa |
| Specificity: | Cdc20 Polyclonal Antibody detects endogenous levels of Cdc20 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human p55CDC at the amino acid range 81-130 |
| Function | Involved in the metaphase/anaphase transition of cell cycle. Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and confers substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. The CDC20-APC/C complex promotes proper dilation formation and radial migration by degrading CCDC41. |
| Protein Name | Cell Division Cycle Protein 20 Homolog P55cdc |
| Database Links | Reactome: R-HSA-141405 Reactome: R-HSA-141430 Reactome: R-HSA-141444 Reactome: R-HSA-174048 Reactome: R-HSA-174113 Reactome: R-HSA-174154 Reactome: R-HSA-174178 Reactome: R-HSA-174184 Reactome: R-HSA-176407 Reactome: R-HSA-176408 Reactome: R-HSA-176409 Reactome: R-HSA-176417 Reactome: R-HSA-179409 Reactome: R-HSA-2467813 Reactome: R-HSA-2500257 Reactome: R-HSA-5663220 Reactome: R-HSA-5689880 Reactome: R-HSA-68877 Reactome: R-HSA-9648025 Reactome: R-HSA-983168 |
| Cellular Localisation | Cytoplasm Cytoskeleton Microtubule Organizing Center Centrosome Chromosome Centromere Kinetochore Spindle Pole |
| Alternative Antibody Names | Anti-Cell Division Cycle Protein 20 Homolog antibody Anti-P55cdc antibody Anti-CDC20 antibody |
Information sourced from Uniprot.org