• Western blot analysis of extracts of Jurkat cells, using CD7 rabbit monoclonal antibody (STJ11102491) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using CD7 rabbit monoclonal antibody (STJ11102491) at dilution of 1:10000 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using CD7 rabbit monoclonal antibody (STJ11102491) at dilution of 1:10000 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD7 antibody (141-240) [S1MR] (STJ11102491)

SKU:
STJ11102491

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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:2000-1:10000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD7
Gene ID: 924
Uniprot ID: CD7_HUMAN
Immunogen Region: 141-240
Specificity: A synthetic peptide corresponding to a sequence within amino acids 141-240 of human CD7 (P09564).
Immunogen Sequence: RCSDAPPRASALPAPPTGSA LPDPQTASALPDPPAASALP AALAVISFLLGLGLGVACVL ARTQIKKLCSWRDKNSAACV VYEDMSHSRCNTLSSPNQYQ
Tissue Specificity Expressed on T-cells and natural killer (NK) cells and their precursors.
Function Transmembrane glycoprotein expressed by T-cells and natural killer (NK) cells and their precursors. Plays a costimulatory role in T-cell activation upon binding to its ligand K12/SECTM1. In turn, mediates the production of cytokines such as IL-2. On resting NK-cells, CD7 activation results in a significant induction of interferon-gamma levels.
Protein Name T-Cell Antigen Cd7
Gp40
T-Cell Leukemia Antigen
T-Cell Surface Antigen Leu-9
Tp41
Cd Antigen Cd7
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-T-Cell Antigen Cd7 antibody
Anti-Gp40 antibody
Anti-T-Cell Leukemia Antigen antibody
Anti-T-Cell Surface Antigen Leu-9 antibody
Anti-Tp41 antibody
Anti-Cd Antigen Cd7 antibody
Anti-CD7 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance