| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CD5 |
| Gene ID: | 921 |
| Uniprot ID: | CD5_HUMAN |
| Immunogen Region: | 158-371 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 158-371 of human CD5 (NP_055022.2). |
| Immunogen Sequence: | RLQLVAQSGGQHCAGVVEFY SGSLGGTISYEAQDKTQDLE NFLCNNLQCGSFLKHLPETE AGRAQDPGEPREHQPLPIQW KIQNSSCTSLEHCFRKIKPQ KSGRVLALLCSGFQPKVQSR LVGGSSICEGTVEVRQGAQW AALCDSSSARSSLRWEEVCR EQQCGSVNSYRVLDAGDPTS RGLFCPHQKLSQCHELWERN SYCKKVFVTCQDPN |
| Post Translational Modifications | Phosphorylated on serine, threonine and tyrosine residues following TCR stimulation. Phosphorylated by LCK on Tyr-453 and Tyr-487 upon TCR engagement. |
| Function | Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL. Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation. Modulates B-cell biology through ERK1/2 activation in a Ca(2+)-dependent pathway via the non-selective Ca(2+) channel TRPC1, leading to IL-10 production. |
| Protein Name | T-Cell Surface Glycoprotein Cd5 Lymphocyte Antigen T1/Leu-1 Cd Antigen Cd5 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-T-Cell Surface Glycoprotein Cd5 antibody Anti-Lymphocyte Antigen T1/Leu-1 antibody Anti-Cd Antigen Cd5 antibody Anti-CD5 antibody Anti-LEU1 antibody |
Information sourced from Uniprot.org