• Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol. Immunofluorescence analysis of rat spleen using CD45RB Rabbit polyclonal antibody (STJ11105069) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol. Immunofluorescence analysis of mouse spleen using CD45RB Rabbit polyclonal antibody (STJ11105069) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Jurkat cells using CD45RB Rabbit polyclonal antibody (STJ11105069) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of Jurkat, using CD45RB Rabbit polyclonal antibody (STJ11105069) at 1:1500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CD45RB antibody (26-463) (STJ11105069)

SKU:
STJ11105069

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD45RB (26-463) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTPRC
Gene ID: 5788
Uniprot ID: PTPRC_HUMAN
Immunogen Region: 26-463
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 26-463 of human CD45RB. (XP_006711537.1).
Immunogen Sequence: QSPTPSPTGVSSVQTPHLPT HADSQTPSAGTDTQTFSGSA ANAKLNPTPGSNAISDAYLN ASETTTLSPSGSAVISTTTI ATTPSKPTCDEKYANITVDY LYNKETKLFTAKLNVNENVE CGNNTCTNNEVHNLTECKNA SVSISHNSCTAPDKTLILDV PPGVEKFQLHDCTQVEKADT TICLKWKNIETFTCDTQNIT YRFQCGNMIFDNKEIKLENL EPEHEYKCDSEILYNNHKF
Tissue Specificity Isoform 1: Detected in thymocytes. Isoform 2: Detected in thymocytes. Isoform 3: Detected in thymocytes. Isoform 4: Not detected in thymocytes. Isoform 5: Detected in thymocytes. Isoform 6: Not detected in thymocytes. Isoform 7: Detected in thymocytes. Isoform 8: Not detected in thymocytes.
Post Translational Modifications Heavily N- and O-glycosylated.
Function Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
Protein Name Receptor-Type Tyrosine-Protein Phosphatase C
Leukocyte Common Antigen
L-Ca
T200
Cd Antigen Cd45
Database Links Reactome: R-HSA-202427
Reactome: R-HSA-416700
Reactome: R-HSA-6798695
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Membrane Raft
Colocalized With Dpp4 In Membrane Rafts
Alternative Antibody Names Anti-Receptor-Type Tyrosine-Protein Phosphatase C antibody
Anti-Leukocyte Common Antigen antibody
Anti-L-Ca antibody
Anti-T200 antibody
Anti-Cd Antigen Cd45 antibody
Anti-PTPRC antibody
Anti-CD45 antibody

Information sourced from Uniprot.org

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