• Western blot analysis of lysate from HeLa cells, using TLR4 antibody.
  • Western blot analysis of COLO205 cells using CD284 Polyclonal Antibody diluted at 1：1000
  • Immunohistochemistry analysis of TLR4 antibody in paraffin-embedded human brain tissue.
  • Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue. 1, CD284 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Western blot analysis of various cells using CD284 Polyclonal Antibody diluted at 1：1000
  • Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1, CD284 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of mouse-spleen tissue. 1, CD284 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of mouse-liver tissue. 1, CD284 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of human-stomach tissue. 1, CD284 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of human-stomach tissue. 1, CD284 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-TLR4 antibody (392-441 aa) (STJ92109)

SKU:
STJ92109

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IF/WB/IHC/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Toll-like receptor 4 (392-441 aa) is suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IF 1:50-200
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TLR4
Gene ID: 7099
Uniprot ID: TLR4_HUMAN
Immunogen Region: 392-441 aa
Specificity: CD284 Polyclonal Antibody detects endogenous levels of CD284 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human CD284 at the amino acid range 392-441
Post Translational Modifications N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS. Phosphorylated on tyrosine residues by LYN after binding lipopolysaccharide.
Function Transmembrane receptor that functions as a pattern recognition receptor recognizing pathogen- and damage-associated molecular patterns (PAMPs and DAMPs) to induce innate immune responses via downstream signaling pathways. At the plasma membrane, cooperates with LY96 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni(2+). Mechanistically, acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Alternatively, CD14-mediated TLR4 internalization via endocytosis is associated with the initiation of a MYD88-independent signaling via the TICAM1-TBK1-IRF3 axis leading to type I interferon production. In addition to the secretion of proinflammatory cytokines, initiates the activation of NLRP3 inflammasome and formation of a positive feedback loop between autophagy and NF-kappa-B signaling cascade. In complex with TLR6, promotes inflammation in monocytes/macrophages by associating with TLR6 and the receptor CD86. Upon ligand binding, such as oxLDL or amyloid-beta 42, the TLR4:TLR6 complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway. In myeloid dendritic cells, vesicular stomatitis virus glycoprotein G but not LPS promotes the activation of IRF7, leading to type I IFN production in a CD14-dependent manner.
Protein Name Toll-Like Receptor 4
Htoll
Cd Antigen Cd284
Database Links Reactome: R-HSA-1236974
Reactome: R-HSA-140534
Reactome: R-HSA-166016
Reactome: R-HSA-166058
Reactome: R-HSA-166166
Reactome: R-HSA-2562578
Reactome: R-HSA-5602498
Reactome: R-HSA-5603041
Reactome: R-HSA-5686938
Reactome: R-HSA-936964
Reactome: R-HSA-937041
Reactome: R-HSA-937072
Reactome: R-HSA-9707616
Reactome: R-HSA-975163
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Early Endosome
Cell Projection
Ruffle
Upon Complex Formation With Cd36 And Tlr6
Internalized Through Dynamin-Dependent Endocytosis
Colocalizes With Rftn1 At Cell Membrane And Then Together With Rftn1 Moves To Endosomes
Upon Lipopolysaccharide Stimulation
Alternative Antibody Names Anti-Toll-Like Receptor 4 antibody
Anti-Htoll antibody
Anti-Cd Antigen Cd284 antibody
Anti-TLR4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance