• Flow cytometric analysis of Raji cells using CD19 monoclonal antibody (green) and negative control (purple).
  • Immunofluorescence analysis of HL-60 (left) and K562 (right) cells using CD19 monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Anti-CD19 antibody [2E2] (STJ97911)

SKU:
STJ97911

Current Stock:
Host: Mouse
Applications: IF/FC/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-B-lymphocyte antigen CD19 is suitable for use in Immunofluorescence, Flow Cytometry and ELISA research applications.
Clonality: Monoclonal
Clone ID: 2E2
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol.
Purification: Affinity purification
Dilution Range: IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD19
Gene ID: 930
Uniprot ID: CD19_HUMAN
Specificity: CD19 Monoclonal Antibody detects endogenous levels of CD19 protein.
Immunogen: Purified recombinant fragment of human CD19 expressed in E. Coli.
Post Translational Modifications Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN. Tyrosine residues are phosphorylated sequentially after activation of the B cell receptor. Phosphorylation of Tyr-531 is extremely rapid, followed by phosphorylation at Tyr-409. In contrast, phosphorylation of Tyr-500 appears more slowly and is more transient, returning rapidly to basal levels.
Function Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores. Is not required for early steps during B cell differentiation in the blood marrow. Required for normal differentiation of B-1 cells. Required for normal B cell differentiation and proliferation in response to antigen challenges. Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge.
Protein Name B-Lymphocyte Antigen Cd19
B-Lymphocyte Surface Antigen B4
Differentiation Antigen Cd19
T-Cell Surface Antigen Leu-12
Cd Antigen Cd19
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-198933
Reactome: R-HSA-2219530
Reactome: R-HSA-6811558
Reactome: R-HSA-977606
Reactome: R-HSA-983695
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Membrane Raft
Alternative Antibody Names Anti-B-Lymphocyte Antigen Cd19 antibody
Anti-B-Lymphocyte Surface Antigen B4 antibody
Anti-Differentiation Antigen Cd19 antibody
Anti-T-Cell Surface Antigen Leu-12 antibody
Anti-Cd Antigen Cd19 antibody
Anti-CD19 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance