• Western blot analysis of extracts of various cell lines, using CD18 antibody (STJ24256) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded Rat lung using CD18 antibody (STJ24256) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Rat spleen using CD18 antibody (STJ24256) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using CD18 antibody (STJ24256) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of THP-1 cells using CD18 rabbit polyclonal antibody (STJ24256) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-937 cells using CD18 rabbit polyclonal antibody (STJ24256) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-ITGB2 antibody (23-125) (STJ24256)

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STJ24256

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD18 (23-125) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ITGB2
Gene ID: 3689
Uniprot ID: ITB2_HUMAN
Immunogen Region: 23-125
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 23-125 of CD18 (NP_000202.3).
Immunogen Sequence: QECTKFKVSSCRECIESGPG CTWCQKLNFTGPGDPDSIRC DTRPQLLMRGCAADDIMDPT SLAETQEDHNGGQKQLSPQK VTLYLRPGQAAAFNVTFRRA KGY
Tissue Specificity Leukocytes. Expressed in neutrophils (at protein level).
Post Translational Modifications Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
Function Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrin ITGAL/ITGB2 is also a receptor for the secreted form of ubiquitin-like protein ISG15.the interaction is mediated by ITGAL. Integrins ITGAM/ITGB2 and ITGAX/ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin ITGAX/ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin ITGAM/ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin ITGAM/ITGB2 is also a receptor for factor X. Integrin ITGAD/ITGB2 is a receptor for ICAM3 and VCAM1. Contributes to natural killer cell cytotoxicity. Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages. In association with alpha subunit ITGAM/CD11b, required for CD177-PRTN3-mediated activation of TNF primed neutrophils.
Protein Name Integrin Beta-2
Cell Surface Adhesion Glycoproteins Lfa-1/Cr3/P150 -95 Subunit Beta
Complement Receptor C3 Subunit Beta
Cd Antigen Cd18
Database Links Reactome: R-HSA-166016
Reactome: R-HSA-198933
Reactome: R-HSA-202733
Reactome: R-HSA-216083
Reactome: R-HSA-6785807
Reactome: R-HSA-6798695
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Membrane Raft
Alternative Antibody Names Anti-Integrin Beta-2 antibody
Anti-Cell Surface Adhesion Glycoproteins Lfa-1/Cr3/P150 -95 Subunit Beta antibody
Anti-Complement Receptor C3 Subunit Beta antibody
Anti-Cd Antigen Cd18 antibody
Anti-ITGB2 antibody
Anti-CD18 antibody
Anti-MFI7 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance