• Immunohistochemistry analysis of CD151 in paraffin-embedded human lung cancer using CD151 Rabbit polyclonal antibody (STJ22974) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CD151 in paraffin-embedded rat kidney using CD151 Rabbit polyclonal antibody (STJ22974) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Rat uterus, using CD151 Rabbit polyclonal antibody (STJ22974) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CD151 antibody (113-253) (STJ22974)

SKU:
STJ22974

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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD151
Gene ID: 977
Uniprot ID: CD151_HUMAN
Immunogen Region: 113-253
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 113-221 of human CD151 (NP_004348.2).
Immunogen Sequence: AYYQQLNTELKENLKDTMTK RYHQPGHEAVTSAVDQLQQE FHCCGSNNSQDWRDSEWIRS QEAGGRVVPDSCCKTVVALC GQRDHASNIYKVEGGCITKL ETFIQEHLR
Tissue Specificity Expressed in a variety of tissues including vascular endothelium and epidermis. Expressed on erythroid cells, with a higher level of expression in erythroid precursors than on mature erythrocytes. Acts as a sensitive T-cell activation marker.
Post Translational Modifications Palmitoylated. Palmitoylation by ZDHHC2 regulates CD151 expression, association with other tetraspanin family proteins and function in cell adhesion. Ubiquitinated by RNF128 on lysine residues present in the tetraspanin amino terminus via 'Lys-48'-linked ubiquitin leading to proteasomal degradation.
Function Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Plays a role in various cellular and molecular mechanism through its association with both integrin and non-integrin proteins. These interactions facilitate critical cellular functions, including cell-to-cell communication, wound healing, platelet aggregation, trafficking, cell motility, and angiogenesis. Via interaction with JAM-A/F11R and integrin ITGA3:ITGB1, promotes the recruitment of signaling molecules such as RAC1, CDC42 and RhoGTPases to facilitate the polarization of epithelial cells and the reorganization of the actin cytoskeleton, which are critical steps in cell migration process. Regulates the glycosylation pattern of ITGA3:ITGB1 thereby modulating its activity. Plays an essential role in the maintenance of central laminin-binding integrin ITGA6:ITGB4-containing adhesion complexes. Essential for the proper assembly of the glomerular and tubular basement membranes in kidney. Contributes to T-cell activation by modulating integrin signaling leading to activation of downstream targets PTK2 and MAPK1/MAPK3. (Microbial infection) Plays a role in human papillomavirus 16/HPV-16 endocytosis upon binding to cell surface receptor. (Microbial infection) Plays a role in human cytomegalovirus entry into host cell by contributing to entry receptor binding, membrane fusion, or release of the capsid.
Protein Name Cd151 Antigen
Gp27
Membrane Glycoprotein Sfa-1
Platelet-Endothelial Tetraspan Antigen 3
Peta-3
Tetraspanin-24
Tspan-24
Cd Antigen Cd151
Database Links Reactome: R-HSA-2022090
Reactome: R-HSA-446107
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Relocalizes To The Immune Synapse In T-Cells Upon Activation
Alternative Antibody Names Anti-Cd151 Antigen antibody
Anti-Gp27 antibody
Anti-Membrane Glycoprotein Sfa-1 antibody
Anti-Platelet-Endothelial Tetraspan Antigen 3 antibody
Anti-Peta-3 antibody
Anti-Tetraspanin-24 antibody
Anti-Tspan-24 antibody
Anti-Cd Antigen Cd151 antibody
Anti-CD151 antibody
Anti-TSPAN24 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance