• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Cyclin C antibody (STJ28628) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using Cyclin C antibody (STJ28628) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using Cyclin C antibody (STJ28628) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Cyclin C antibody (STJ28628) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CCNC antibody (1-283) (STJ28628)

SKU:
STJ28628

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CCNC (1-283) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCNC
Gene ID: 892
Uniprot ID: CCNC_HUMAN
Immunogen Region: 1-283
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human Cyclin C (NP_005181.2).
Immunogen Sequence: MAGNFWQSSHYLQWILDKQD LLKERQKDLKFLSEEEYWKL QIFFTNVIQALGEHLKLRQQ VIATATVYFKRFYARYSLKS IDPVLMAPTCVFLASKVEEF GVVSNTRLIAAATSVLKTRF SYAFPKEFPYRMNHILECEF YLLELMDCCLIVYHPYRPLL QYVQDMGQEDMLLPLAWRIV NDTYRTDLCLLYPPFMIALA CLHVACVVQQKDARQWFAEL SVDMEKILEIIRVILKLYE
Tissue Specificity Highest levels in pancreas. High levels in heart, liver, skeletal muscle and kidney. Low levels in brain.
Function Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex.
Protein Name Cyclin-C
Srb11 Homolog
Hsrb11
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-2122947
Reactome: R-HSA-212436
Reactome: R-HSA-2173796
Reactome: R-HSA-2644606
Reactome: R-HSA-2894862
Reactome: R-HSA-381340
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Cyclin-C antibody
Anti-Srb11 Homolog antibody
Anti-Hsrb11 antibody
Anti-CCNC antibody

Information sourced from Uniprot.org

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