![Western blot analysis of lysates from wild type (WT) and Catalasealase knockout (KO) HeLa cells, using [KO Validated] Catalase Rabbit polyclonal antibody (STJ119991) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98776/382824/STJ119991_1__14020.1713151324.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Catalasealase knockout (KO) HeLa cells, using [KO Validated] Catalase Rabbit polyclonal antibody (STJ119991) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western blot analysis of lysates from wild type (WT) and Catalasealase knockout (KO) HeLa cells, using [KO Validated] Catalase Rabbit polyclonal antibody (STJ119991) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/98776/382824/STJ119991_1__14020.1713151324.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Catalasealase knockout (KO) HeLa cells, using [KO Validated] Catalase Rabbit polyclonal antibody (STJ119991) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:3000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CAT |
| Gene ID: | 847 |
| Uniprot ID: | CATA_HUMAN |
| Immunogen Region: | 1-225 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human Catalasealase (NP_001743.1). |
| Immunogen Sequence: | MADSRDPASDQMQHWKEQRA AQKADVLTTGAGNPVGDKLN VITVGPRGPLLVQDVVFTDE MAHFDRERIPERVVHAKGAG AFGYFEVTHDITKYSKAKVF EHIGKKTPIAVRFSTVAGES GSADTVRDPRGFAVKFYTED GNWDLVGNNTPIFFIRDPIL FPSFIHSQKRNPQTHLKDPD MVWDFWSLRPESLHQVSFLF SDRGIPDGHRHMNGYGSHTF KLVNA |
| Function | Catalyzes the degradation of hydrogen peroxide (H(2)O(2)) generated by peroxisomal oxidases to water and oxygen, thereby protecting cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. |
| Protein Name | Catalase |
| Database Links | Reactome: R-HSA-3299685Reactome: R-HSA-6798695Reactome: R-HSA-9033241Reactome: R-HSA-9615017 |
| Cellular Localisation | Peroxisome Matrix |
| Alternative Antibody Names | Anti-Catalase antibodyAnti-CAT antibody |
Information sourced from Uniprot.org
