• Immunohistochemistry analysis of paraffin-embedded mouse brain using CAST antibody (STJ22910) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human prostate using CAST antibody (STJ22910) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using CAST antibody (STJ22910) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of H460 cells, using CAST antibody (STJ22910) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CAST antibody (677-695) (STJ22910)

SKU:
STJ22910

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CAST (677-695) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CAST
Gene ID: 831
Uniprot ID: ICAL_HUMAN
Immunogen Region: 677-695
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 677-695 of human CAST (NP_001177371.1).
Immunogen Sequence: GKAKDSAKTTEETSKPKDD
Post Translational Modifications The N-terminus is blocked.
Function Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
Protein Name Calpastatin
Calpain Inhibitor
Sperm Bs-17 Component
Database Links Reactome: R-HSA-1474228
Reactome: R-HSA-8862803
Alternative Antibody Names Anti-Calpastatin antibody
Anti-Calpain Inhibitor antibody
Anti-Sperm Bs-17 Component antibody
Anti-CAST antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance