• Western blot analysis of lysates from NIH/3T3 cells, treated with TNF-a 20ng/ml 30', using Caspase 9 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Caspase 9 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of HUVEC cells, using Caspase 9 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of 3T3 cells using Caspase-9 Polyclonal Antibody diluted at 1：500
  • Western blot analysis of various cells using Caspase-9 Polyclonal Antibody diluted at 1：500

Anti-CASP9 antibody (91-140 aa) (STJ92027)

SKU:
STJ92027

€60.00 - €315.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Caspase-9 (91-140 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CASP9
Gene ID: 842
Uniprot ID: CASP9_HUMAN
Immunogen Region: 91-140 aa
Specificity: Caspase-9 Polyclonal Antibody detects endogenous levels of Caspase-9 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Caspase 9 at the amino acid range 91-140
Post Translational Modifications Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl.occurs in the response of cells to DNA damage. (Microbial infection) ADP-riboxanation by C.violaceum CopC blocks CASP9 processing, preventing CASP9 activation and ability to mediate intrinsic apoptosis. Ubiquitinated by BIRC6.this activity is inhibited by DIABLO/SMAC.
Function Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC. Isoform 2: Lacks activity is an dominant-negative inhibitor of caspase-9.
Protein Name Caspase-9
Casp-9
Apoptotic Protease Mch-6
Apoptotic Protease-Activating Factor 3
Apaf-3
Ice-Like Apoptotic Protease 6
Ice-Lap6 Cleaved Into - Caspase-9 Subunit P35 - Caspase-9 Subunit P10
Database Links Reactome: R-HSA-111458
Reactome: R-HSA-111459
Reactome: R-HSA-111463
Reactome: R-HSA-111464
Reactome: R-HSA-168638
Reactome: R-HSA-198323
Reactome: R-HSA-418889
Reactome: R-HSA-5674400
Reactome: R-HSA-9627069
Alternative Antibody Names Anti-Caspase-9 antibody
Anti-Casp-9 antibody
Anti-Apoptotic Protease Mch-6 antibody
Anti-Apoptotic Protease-Activating Factor 3 antibody
Anti-Apaf-3 antibody
Anti-Ice-Like Apoptotic Protease 6 antibody
Anti-Ice-Lap6 Cleaved Into - Caspase-9 Subunit P35 - Caspase-9 Subunit P10 antibody
Anti-CASP9 antibody
Anti-MCH6 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance