• Immunohistochemistry analysis of paraffin-embedded rat brain using CARS Rabbit monoclonal antibody (STJ11102681) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CARS antibody (STJ11102681) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-CARS antibody (650-748) [S1MR] (STJ11102681)

SKU:
STJ11102681

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CARS (650-748) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CARS1
Gene ID: 833
Uniprot ID: SYCC_HUMAN
Immunogen Region: 650-748
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 650-748 of human CARS (P49589).
Immunogen Sequence: EREEKRRVEEEKRKKKEEAA RRKQEQEAAKLAKMKIPPSE MFLSETDKYSKFDENGLPTH DMEGKELSKGQAKKLKKLFE AQEKLYKEYLQMAQNGSFQ
Function Catalyzes the ATP-dependent ligation of cysteine to tRNA(Cys).
Protein Name Cysteine--Trna Ligase - Cytoplasmic
Cysteinyl-Trna Synthetase
Cysrs
Database Links Reactome: R-HSA-379716
Reactome: R-HSA-9725370
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Cysteine--Trna Ligase - Cytoplasmic antibody
Anti-Cysteinyl-Trna Synthetase antibody
Anti-Cysrs antibody
Anti-CARS1 antibody
Anti-CARS antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance