• Immunohistochemistry analysis of paraffin-embedded human liver cancer using CAPG Antibody (STJ29463) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using CAPG Antibody (STJ29463) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts from normal (control) and CAPG knockout (KO) HeLa cells, using CAPG antibody (STJ29463) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using CAPG Antibody (STJ29463) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using CAPG Antibody (STJ29463) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CAPG antibody (1-348) (STJ29463)

SKU:
STJ29463

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CAPG (1-348) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CAPG
Gene ID: 822
Uniprot ID: CAPG_HUMAN
Immunogen Region: 1-348
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-348 of human CAPG (NP_001738.2).
Immunogen Sequence: MYTAIPQSGSPFPGSVQDPG LHVWRVEKLKPVPVAQENQG VFFSGDSYLVLHNGPEEVSH LHLWIGQQSSRDEQGACAVL AVHLNTLLGERPVQHREVQG NESDLFMSYFPRGLKYQEGG VESAFHKTSTGAPAAIKKLY QVKGKKNIRATERALNWDSF NTGDCFILDLGQNIFAWCGG KSNILERNKARDLALAIRDS ERQGKAQVEIVTDGEEPAEM IQVLGPKPALKEGNPEEDL
Tissue Specificity Macrophages and macrophage-like cells.
Post Translational Modifications The N-terminus is blocked.
Function Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.
Protein Name Macrophage-Capping Protein
Actin Regulatory Protein Cap-G
Cellular Localisation Nucleus
Cytoplasm
Melanosome
Cell Projection
Lamellipodium
Ruffle
In Macrophages
May Be Predominantly Cytoplasmic
Nuclear Localization Was Observed In Fibroblasts
Present At The Membrane-Cytoplasm Interface
In Activated Macrophages
Concentrated In The Ruffles Of The Leading Lamellipodia
Alternative Antibody Names Anti-Macrophage-Capping Protein antibody
Anti-Actin Regulatory Protein Cap-G antibody
Anti-CAPG antibody
Anti-AFCP antibody
Anti-MCP antibody

Information sourced from Uniprot.org

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