• Western blot analysis of extracts of various cell lines, using (STJ28263) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CANT1 antibody (62-231) (STJ28263)

SKU:
STJ28263

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CANT1 (62-231) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CANT1
Gene ID: 124583
Uniprot ID: CANT1_HUMAN
Immunogen Region: 62-231
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 62-231 of human CANT1 (NP_620148.1).
Immunogen Sequence: HRPAPGRPPTHNAHNWRLGQ APANWYNDTYPLSPPQRTPA GIRYRIAVIADLDTESRAQE ENTWFSYLKKGYLTLSDSGD KVAVEWDKDHGVLESHLAEK GRGMELSDLIVFNGKLYSVD DRTGVVYQIEGSKAVPWVIL SDGDGTVEKGFKAEWLAVKD ERLYVGGLGK
Tissue Specificity Widely expressed.
Post Translational Modifications N-glycosylated.
Function Calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > UTP > GTP. Has very low activity towards ADP and even lower activity towards ATP. Does not hydrolyze AMP and GMP. Involved in proteoglycan synthesis.
Protein Name Soluble Calcium-Activated Nucleotidase 1
Scan-1
Apyrase Homolog
Putative Mapk-Activating Protein Pm09
Putative Nf-Kappa-B-Activating Protein 107
Database Links Reactome: R-HSA-6798695
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Ii Membrane Protein
Golgi Apparatus
Golgi Stack Membrane
Cell Membrane
Processed Form: Secreted
Alternative Antibody Names Anti-Soluble Calcium-Activated Nucleotidase 1 antibody
Anti-Scan-1 antibody
Anti-Apyrase Homolog antibody
Anti-Putative Mapk-Activating Protein Pm09 antibody
Anti-Putative Nf-Kappa-B-Activating Protein 107 antibody
Anti-CANT1 antibody
Anti-SHAPY antibody

Information sourced from Uniprot.org

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