Anti-CaMKII beta antibody [R05-9G5] (STJA0034104)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human CaMKII
General Information
| Short Description | Rabbit monoclonal anti-CaMKII beta for use in WB in Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R05-9G5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | CAMK2B |
| Gene ID | 816 |
| Uniprot ID | KCC2B_HUMAN |
| Immunogen | A synthetic peptide of human CaMKII |
Additional Info
| Tissue Specificity | Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle. |
| Post Translational Modifications | Autophosphorylation of Thr-287 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-287 locks the kinase into an activated state. |
| Function | Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca(2+) transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Also regulates the migration of developing neurons. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2. In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway. Phosphorylates reticulophagy regulator RETREG1 at 'Ser-151' under endoplasmic reticulum stress conditions which enhances RETREG1 oligomerization and its membrane scission and reticulophagy activity. |
| Protein Name | Calcium/Calmodulin-Dependent Protein Kinase Type Ii Subunit BetaCam Kinase Ii Subunit BetaCamk-Ii Subunit Beta |
| Database Links | Reactome: R-HSA-111932Reactome: R-HSA-3371571Reactome: R-HSA-399719Reactome: R-HSA-438066Reactome: R-HSA-442729Reactome: R-HSA-442982Reactome: R-HSA-5576892Reactome: R-HSA-5578775Reactome: R-HSA-5673000Reactome: R-HSA-5673001Reactome: R-HSA-6802946Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-877300Reactome: R-HSA-9022692Reactome: R-HSA-936837Reactome: R-HSA-9609736Reactome: R-HSA-9617324Reactome: R-HSA-9620244Reactome: R-HSA-9649948Reactome: R-HSA-9656223 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeSarcoplasmic Reticulum MembranePeripheral Membrane ProteinCytoplasmic SideSynapseIn Slow-Twitch MuscleEvenly Distributed Between Longitudinal Sr And Junctional Sr |
| Alternative Antibody Names | Anti-Calcium/Calmodulin-Dependent Protein Kinase Type Ii Subunit Beta antibodyAnti-Cam Kinase Ii Subunit Beta antibodyAnti-Camk-Ii Subunit Beta antibodyAnti-CAMK2B antibodyAnti-CAM2 antibodyAnti-CAMK2 antibodyAnti-CAMKB antibody |
Information sourced from Uniprot.org