• Western blot analysis of lysates from COLO cells, using CaMK1-alpha Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using CaMK1-alpha Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-CAMK1 antibody (143-192 aa) (STJ91993)

SKU:
STJ91993

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Calcium/calmodulin-dependent protein kinase type 1 (143-192 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CAMK1
Gene ID: 8536
Uniprot ID: KCC1A_HUMAN
Immunogen Region: 143-192 aa
Specificity: CaMKI Alpha Polyclonal Antibody detects endogenous levels of CaMKI Alpha protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human CaMK1-alpha at the amino acid range 143-192
Post Translational Modifications Phosphorylated by CaMKK1 and CaMKK2 on Thr-177. Polybiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL12), leading to proteasomal degradation.
Function Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence MVLIF-x-R-x(2)-ST-x(3)-MVLIF. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca(2+) elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser-694', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser-498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen-stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation. Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K(+) and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I.
Protein Name Calcium/Calmodulin-Dependent Protein Kinase Type 1
Cam Kinase I
Cam-Ki
Cam Kinase I Alpha
Camki-Alpha
Database Links Reactome: R-HSA-9617324
Reactome: R-HSA-9619229
Cellular Localisation Cytoplasm
Nucleus
Predominantly Cytoplasmic
Alternative Antibody Names Anti-Calcium/Calmodulin-Dependent Protein Kinase Type 1 antibody
Anti-Cam Kinase I antibody
Anti-Cam-Ki antibody
Anti-Cam Kinase I Alpha antibody
Anti-Camki-Alpha antibody
Anti-CAMK1 antibody

Information sourced from Uniprot.org

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