• Western blot analysis of extracts of Rat heart, using Calsequestrin 1 rabbit monoclonal antibody (STJ11103065) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of mouse skeletal muscle, using Calsequestrin 1 rabbit monoclonal antibody (STJ11103065) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of mouse skeletal muscle using Calsequestrin 1 antibody (STJ11103065) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Rat skeletal muscle using Calsequestrin 1 antibody (STJ11103065) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-Calsequestrin 1 antibody (100-200) [S5MR] (STJ11103065)

SKU:
STJ11103065

£129.50 - £574.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CASQ1
Gene ID: 844
Uniprot ID: CASQ1_HUMAN
Immunogen Region: 100-200
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human Calsequestrin 1 (NP_001222.3).
Immunogen Sequence: EDKGVGFGLVDSEKDAAVAK KLGLTEVDSMYVFKGDEVIE YDGEFSADTIVEFLLDVLED PVELIEGERELQAFENIEDE IKLIGYFKSKDSEHYKAFED A
Tissue Specificity Expressed in myoblasts (at protein level).
Post Translational Modifications N-glycosylated.
Function Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. Calcium ions are bound by clusters of acidic residues at the protein surface, often at the interface between subunits. Can bind around 80 Ca(2+) ions. Regulates the release of lumenal Ca(2+) via the calcium release channel RYR1.this plays an important role in triggering muscle contraction. Negatively regulates store-operated Ca(2+) entry (SOCE) activity.
Protein Name Calsequestrin-1
Calmitine
Calsequestrin - Skeletal Muscle Isoform
Database Links Reactome: R-HSA-2672351
Reactome: R-HSA-5578775
Cellular Localisation Endoplasmic Reticulum
Sarcoplasmic Reticulum
Sarcoplasmic Reticulum Lumen
Sarcoplasmic Reticulum Membrane
Peripheral Membrane Protein
Lumenal Side
Mitochondrion Matrix
This Isoform Of Calsequestrin Occurs In The Sarcoplasmic Reticulum's Terminal Cisternae Luminal Spaces Of Fast Skeletal Muscle Cells
Preferentially Forms Linear And Round Aggregates In The Endoplasmic Reticulum (Er) Of Resting Cells
In A Minority Of Cells
Homogeneously Detected In The Er Lumen
Colocalizes With Stim1 At Endoplasmic Reticulum In Response To A Depletion Of Intracellular Calcium
Alternative Antibody Names Anti-Calsequestrin-1 antibody
Anti-Calmitine antibody
Anti-Calsequestrin - Skeletal Muscle Isoform antibody
Anti-CASQ1 antibody
Anti-CASQ antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance