Western blot analysis of extracts of various cell lines, using CALCRL antibody (STJ111275) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-CALCRL antibody (430-461) (STJ111275)

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STJ111275
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CALCRL
Gene ID: 10203
Uniprot ID: CALRL_HUMAN
Immunogen Region: 430-461
Specificity: A synthetic peptide corresponding to a sequence within amino acids 346-445 of human CALCRL (NP_005786.1).
Immunogen Sequence: GIEFVLIPWRPEGKIAEEVY DYIMHILMHFQGLLVSTIFC FFNGEVQAILRRNWNQYKIQ FGNSFSNSEALRSASYTVST ISDGPGYSHDCPSEHLNGKS IHDIENVLLKPENLYN
Tissue Specificity Predominantly expressed in the lung and heart.
Function G protein-coupled receptor which specificity is determined by its interaction with receptor-activity-modifying proteins (RAMPs). Together with RAMP1, form the receptor complex for calcitonin-gene-related peptides CALCA/CGRP1 and CALCB/CGRP2. Together with RAMP2 or RAMP3, function as receptor complexes for adrenomedullin (ADM and ADM2). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. Activates cAMP-dependent pathway.
Protein Name Calcitonin Gene-Related Peptide Type 1 Receptor
Cgrp Type 1 Receptor
Calcitonin Receptor-Like Receptor
Crlr
Database Links Reactome: R-HSA-418555
Reactome: R-HSA-419812
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Calcitonin Gene-Related Peptide Type 1 Receptor antibody
Anti-Cgrp Type 1 Receptor antibody
Anti-Calcitonin Receptor-Like Receptor antibody
Anti-Crlr antibody
Anti-CALCRL antibody
Anti-CGRPR antibody

Information sourced from Uniprot.org

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