• Western blot analysis of extracts of various cell lines, using Carbonic Anhydrase 2 (CA2) rabbit monoclonal antibody (STJ11102438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunohistochemistry analysis of Carbonic Anhydrase 2 (CA2) in paraffin-embedded human colon tissue using Carbonic Anhydrase 2 (CA2) rabbit monoclonal antibody (STJ11102438) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Carbonic Anhydrase 2 (CA2) in paraffin-embedded mouse brain tissue using Carbonic Anhydrase 2 (CA2) rabbit monoclonal antibody (STJ11102438) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Carbonic Anhydrase 2 (CA2) in paraffin-embedded mouse kidney tissue using Carbonic Anhydrase 2 (CA2) rabbit monoclonal antibody (STJ11102438) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Carbonic Anhydrase 2 (CA2) in paraffin-embedded rat brain tissue using Carbonic Anhydrase 2 (CA2) rabbit monoclonal antibody (STJ11102438) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Carbonic Anhydrase 2 (CA2) in paraffin-embedded rat colon tissue using Carbonic Anhydrase 2 (CA2) rabbit monoclonal antibody (STJ11102438) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Carbonic Anhydrase 2 (CA2) in paraffin-embedded rat kidney tissue using Carbonic Anhydrase 2 (CA2) rabbit monoclonal antibody (STJ11102438) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.

Anti-CA2 antibody (161-260) [S8MR] (STJ11102438)

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STJ11102438

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Carbonic anhydrase 2 (161-260) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CA2
Gene ID: 760
Uniprot ID: CAH2_HUMAN
Immunogen Region: 161-260
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 161-260 of human Carbonic Anhydrase 2 (CA2) (P00918).
Immunogen Sequence: DVLDSIKTKGKSADFTNFDP RGLLPESLDYWTYPGSLTTP PLLECVTWIVLKEPISVSSE QVLKFRKLNFNGEGEPEELM VDNWRPAQPLKNRQIKASFK
Function Catalyzes the reversible hydration of carbon dioxide. Can also hydrate cyanamide to urea. Stimulates the chloride-bicarbonate exchange activity of SLC26A6. Essential for bone resorption and osteoclast differentiation. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption.
Protein Name Carbonic Anhydrase 2
Carbonate Dehydratase Ii
Carbonic Anhydrase C
Cac
Carbonic Anhydrase Ii
Ca-Ii
Cyanamide Hydratase Ca2
Database Links Reactome: R-HSA-1237044
Reactome: R-HSA-1247673
Reactome: R-HSA-1475029
Cellular Localisation Cytoplasm
Cell Membrane
Colocalized With Slc26a6 At The Surface Of The Cell Membrane In Order To Form A Bicarbonate Transport Metabolon
Displaced From The Cytosolic Surface Of The Cell Membrane By Pkc In Phorbol Myristate Acetate (Pma)-Induced Cells
Alternative Antibody Names Anti-Carbonic Anhydrase 2 antibody
Anti-Carbonate Dehydratase Ii antibody
Anti-Carbonic Anhydrase C antibody
Anti-Cac antibody
Anti-Carbonic Anhydrase Ii antibody
Anti-Ca-Ii antibody
Anti-Cyanamide Hydratase Ca2 antibody
Anti-CA2 antibody

Information sourced from Uniprot.org

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