• Western blot analysis of extracts of HT-29 cells, using CA12 antibody (STJ112225) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 30s.

Anti-CA12 antibody (25-301) (STJ112225)

SKU:
STJ112225

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CA12 (25-301) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CA12
Gene ID: 771
Uniprot ID: CAH12_HUMAN
Immunogen Region: 25-301
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-301 of human CA12 (NP_001209.1).
Immunogen Sequence: APVNGSKWTYFGPDGENSWS KKYPSCGGLLQSPIDLHSDI LQYDASLTPLEFQGYNLSAN KQFLLTNNGHSVKLNLPSDM HIQGLQSRYSATQLHLHWGN PNDPHGSEHTVSGQHFAAEL HIVHYNSDLYPDASTASNKS EGLAVLAVLIEMGSFNPSYD KIFSHLQHVKYKGQEAFVPG FNIEELLPERTAEYYRYRGS LTTPPCNPTVLWTVFRNPVQ ISQEQLLALETALYCTHMD
Tissue Specificity Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis. Expressed in sweat glands and bronchiolar epithelium.
Function Reversible hydration of carbon dioxide.
Protein Name Carbonic Anhydrase 12
Carbonate Dehydratase Xii
Carbonic Anhydrase Xii
Ca-Xii
Tumor Antigen Hom-Rcc-3.1.3
Database Links Reactome: R-HSA-1475029
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Cell Membrane
Alternative Antibody Names Anti-Carbonic Anhydrase 12 antibody
Anti-Carbonate Dehydratase Xii antibody
Anti-Carbonic Anhydrase Xii antibody
Anti-Ca-Xii antibody
Anti-Tumor Antigen Hom-Rcc-3.1.3 antibody
Anti-CA12 antibody

Information sourced from Uniprot.org

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