Host: | Rabbit |
Applications: | WB/IHC/IF/ELISA |
Reactivity: | Human/Rat/Mouse |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description : | Rabbit polyclonal antibody anti-Complement C4-A and Complement C4-B (721-770 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
Clonality : | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Concentration: | 1 mg/mL |
Dilution Range: | WB 1:500-1:2000 IHC-P 1:100-1:300 ELISA 1:10000 IF 1:50-200 |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | C4B.C4B_2 C4A |
Gene ID: | 100293534/721 720/721 |
Uniprot ID: | CO4B_HUMAN CO4A_HUMAN |
Immunogen Region: | 721-770 aa |
Specificity: | C4a/b Polyclonal Antibody detects endogenous levels of C4a/b protein. |
Immunogen: | The antiserum was produced against synthesized peptide derived from the Internal region of human C4A/C4B at the amino acid range 721-770 |
Post Translational Modifications | Prior to secretion, the single-chain precursor is enzymatically cleaved to yield non-identical chains alpha, beta and gamma. During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase. The proteolytic cleavages often are incomplete so that many structural forms can be found in plasma. N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. |
Function | Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens. Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. |
Protein Name | Complement C4-A Acidic Complement C4 C3 And Pzp-Like Alpha-2-Macroglobulin Domain-Containing Protein 2 Cleaved Into - Complement C4 Beta Chain - Complement C4-A Alpha Chain - C4a Anaphylatoxin - C4b-A - C4d-A - Complement C4 Gamma Chain |
Database Links | Reactome: R-HSA-166663 Reactome: R-HSA-174577 Reactome: R-HSA-381426 Reactome: R-HSA-8957275 Reactome: R-HSA-977606 |
Cellular Localisation | Secreted Cell Junction Synapse Cell Projection Axon Dendrite |
Alternative Antibody Names | Anti-Complement C4-A antibody Anti-Acidic Complement C4 antibody Anti-C3 And Pzp-Like Alpha-2-Macroglobulin Domain-Containing Protein 2 Cleaved Into - Complement C4 Beta Chain - Complement C4-A Alpha Chain - C4a Anaphylatoxin - C4b-A - C4d-A - Complement C4 Gamma Chain antibody Anti-C4A antibody Anti-CO4 antibody Anti-CPAMD2 antibody |
Information sourced from Uniprot.org