Anti-C1R antibody (466-705) (STJ28443)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal C1R (466-705) antibody for use in IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | C1R |
| Gene ID | 715 |
| Uniprot ID | C1R_HUMAN |
| Immunogen Region | 466-705 |
| Immunogen Sequence | GGQKAKMGNFPWQVFTNIHG RGGGALLGDRWILTAAHTLY PKEHEAQSNASLDVFLGHTN VEELMKLGNHPIRRVSVHPD YRQDESYNFEGDIALLELEN SVTLGPNLLPICLPDNDTFY DLGLMGYVSGFGVMEEKIAH DLRFVRLPVANPQACENWLR GKNRMDVFSQNMFCAGHPSL KQDACQGDSGGVFAVRDPNT DRWVATGIVSWGIGCSRGYG FYTKVLNYVDWIKKEMEEE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 466-705 of human C1R (NP_001724.3). |
Additional Info
| Post Translational Modifications | Cleaved and activated by autocatalytic processing to generate Complement C1r subcomponent heavy and light chains that are connected by disulfide bonds. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. |
| Function | Serine protease component of the complement C1 complex, a multiprotein complex that initiates the classical pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. C1R catalyzes the first enzymatic step in the classical complement pathway: it is activated by the C1Q subcomplex of the C1 complex, which associates with IgG or IgM immunoglobulins complexed with antigens to form antigen-antibody complexes on the surface of pathogens. Immunoglobulin-binding promotes the autocatalytic cleavage and activation of C1R. Activated C1R then cleaves and activates C1S, the second protease of the classical complement pathway. It is unclear if C1R activates C1S within single, strained C1 complexes or between neighboring C1 complexes on surfaces. |
| Protein Name | Complement C1r SubcomponentComplement Component 1 Subcomponent R Cleaved Into - Complement C1r Subcomponent Heavy ChainComplement C1r Subcomponent Chain A - Complement C1r Subcomponent Light ChainComplement C1r Subcomponent Chain B |
| Database Links | Reactome: R-HSA-166663Reactome: R-HSA-173623Reactome: R-HSA-977606 |
| Cellular Localisation | SecretedCell SurfaceRecruited To The Surface Of Pathogens By The C1q Subcomplex |
| Alternative Antibody Names | Anti-Complement C1r Subcomponent antibodyAnti-Complement Component 1 Subcomponent R Cleaved Into - Complement C1r Subcomponent Heavy Chain antibodyAnti-Complement C1r Subcomponent Chain A - Complement C1r Subcomponent Light Chain antibodyAnti-Complement C1r Subcomponent Chain B antibodyAnti-C1R antibody |
Information sourced from Uniprot.org