• Immunohistochemistry of paraffin-embedded mouse kidney using Histone H2A Rabbit mAb (STJ11101853) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human brain using Histone H2A Rabbit mAb (STJ11101853) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of Rat liver, using Histone H2A Rabbit mAb (STJ11101853) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3min.
  • Immunohistochemistry of paraffin-embedded rat lung using Histone H2A Rabbit mAb (STJ11101853) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using Histone H2A Rabbit mAb (STJ11101853) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.

Anti-c-Maf antibody (STJ11101853)

SKU:
STJ11101853
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human, Mouse, Rat
Short Description: Rabbit monoclonal primary antibody against MAF.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Monoclonal
Clone ID: ARC1954
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
Uniprot ID: MAF_HUMAN
Gene ID: 4094
Gene Symbol: MAF
Immunogen: A synthesized peptide derived from human c-Maf
Tissue Specificity Expressed in endothelial cells.
Post Translational Modifications Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is triggered by glucocorticoids. Phosphorylated by GSK3 and MAPK13 on serine and threonine residues (Probable). The phosphorylation status can serve to either stimulate or inhibit transcription.
Function Acts as a transcriptional activator or repressor. Involved in embryonic lens fiber cell development. Recruits the transcriptional coactivators CREBBP and/or EP300 to crystallin promoters leading to up-regulation of crystallin gene during lens fiber cell differentiation. Activates the expression of IL4 in T helper 2 (Th2) cells. Increases T-cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, transactivates strongly the glucagon gene promoter through the G1 element. Activates transcription of the CD13 proximal promoter in endothelial cells. Represses transcription of the CD13 promoter in early stages of myelopoiesis by affecting the ETS1 and MYB cooperative interaction. Involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. Binds to the sequence 5'-GTGGCNGTNCTCAGNN-3' in the L7 promoter. Binds to the T-MARE (Maf response element) sites of lens-specific alpha- and beta-crystallin gene promoters. Binds element G1 on the glucagon promoter. Binds an AT-rich region adjacent to the TGC motif (atypical Maf response element) in the CD13 proximal promoter in endothelial cells. When overexpressed, represses anti-oxidant response element (ARE)-mediated transcription. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Binds to the ARE sites of detoxifying enzyme gene promoters.
Database Links Reactome: R-HSA-8940973
Cellular Localisation Nucleus
Protein Name Transcription Factor Maf
Proto-Oncogene C-Maf
V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog
Alternative Names Anti-Transcription Factor Maf antibody
Anti-Proto-Oncogene C-Maf antibody
Anti-V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog antibody
Anti-MAF antibody
6 months standard