• Immunofluorescence analysis of NIH/3T3 cells using c-Jun antibody (STJ24273) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using c-Jun antibody (STJ24273) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

Anti-c-Jun antibody (STJ24273)

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STJ24273
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Host: Rabbit
Applications: WB, IF
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal primary antibody against JUN.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IF: 1:50-1:200
Uniprot ID: JUN_HUMAN
Gene ID: 3725
Gene Symbol: JUN
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human c-Jun (NP_002219.1).
Background This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
Tissue Specificity Expressed in the developing and adult prostate and prostate cancer cells.
Function Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGACGTCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells. Binds to the USP28 promoter in colorectal cancer (CRC) cells.
Database Links Reactome: R-HSA-1912408
Cellular Localisation Nucleus
Protein Name Transcription Factor Ap-1
Activator Protein 1
Ap1
Proto-Oncogene C-Jun
V-Jun Avian Sarcoma Virus 17 Oncogene Homolog
P39
Alternative Names Anti-Transcription Factor Ap-1 antibody
Anti-Activator Protein 1 antibody
Anti-Ap1 antibody
Anti-Proto-Oncogene C-Jun antibody
Anti-V-Jun Avian Sarcoma Virus 17 Oncogene Homolog antibody
Anti-P39 antibody
Anti-JUN antibody

Information sourced from uniprot.org

6 months standard