• Western blot analysis of lysates from HT29 cells, using CSFR Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CSFR Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of HT29 cells using c-Fms Polyclonal Antibody
  • Immunofluorescence analysis of HeLa cells, using CSFR Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using c-Fms Polyclonal Antibody

Anti-c-Fms antibody (STJ92246)

SKU:
STJ92246
Current Stock:
Host: Rabbit
Applications: WB, IHC-P, IF, ELISA
Reactivity: Human
Short Description: Rabbit polyclonal primary antibody against c-Fms.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Specificity: c-Fms polyclonal antibody detects endogenous levels of c-Fms protein.
Uniprot ID: CSF1R_HUMAN
Gene ID: 1436
Gene Symbol: CSF1R
Immunogen: Synthesized peptide derived from human c-Fms around the non-phosphorylation site of Y809.
Immunogen Region: 750-830 aa
Tissue Specificity Expressed in bone marrow and in differentiated blood mononuclear cells.
Post Translational Modifications Autophosphorylated in response to CSF1 or IL34 binding. Phosphorylation at Tyr-561 is important for normal down-regulation of signaling by ubiquitination, internalization and degradation. Phosphorylation at Tyr-561 and Tyr-809 is important for interaction with SRC family members, including FYN, YES1 and SRC, and for subsequent activation of these protein kinases. Phosphorylation at Tyr-699 and Tyr-923 is important for interaction with GRB2. Phosphorylation at Tyr-723 is important for interaction with PIK3R1. Phosphorylation at Tyr-708 is important for normal receptor degradation. Phosphorylation at Tyr-723 and Tyr-809 is important for interaction with PLCG2. Phosphorylation at Tyr-969 is important for interaction with CBL. Dephosphorylation by PTPN2 negatively regulates downstream signaling and macrophage differentiation. Ubiquitinated. Becomes rapidly polyubiquitinated after autophosphorylation, leading to its degradation.
Function Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway. Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages.
Database Links Reactome: R-HSA-449836
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Protein Name Macrophage Colony-Stimulating Factor 1 Receptor
Csf-1 Receptor
Csf-1-R
Csf-1r
M-Csf-R
Proto-Oncogene C-Fms
Cd Antigen Cd115
Alternative Names Anti-Macrophage Colony-Stimulating Factor 1 Receptor antibody
Anti-Csf-1 Receptor antibody
Anti-Csf-1-R antibody
Anti-Csf-1r antibody
Anti-M-Csf-R antibody
Anti-Proto-Oncogene C-Fms antibody
Anti-Cd Antigen Cd115 antibody
Anti-CSF1R antibody
Anti-FMS antibody
6 months standard