Anti-BRSK2/STK29 antibody (Internal) (STJ70801)

SKU:
STJ70801-100

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Host: Goat
Applications: Pep-ELISA/FC/IF
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-BRSK2/STK29 (Internal) is suitable for use in ELISA, Flow Cytometry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: ELISA-antibody detection limit dilution 1:8000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: BRSK2
Gene ID: 9024
Uniprot ID: BRSK2_HUMAN
Immunogen Region: Internal
Accession Number: NP_003948.2
Immunogen Sequence: CPEVIRGEKYDGRKAD
Post Translational Modifications Phosphorylated at Thr-174 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Not phosphorylated at Thr-174 by CaMKK2. In contrast, it is phosphorylated and activated by CaMKK1. May be inactivated via dephosphorylation of Thr-174 by PP2C. Phosphorylated at Thr-260 by PKA. Phosphorylation at Thr-260 by PKA was not observed in another study, but this may reflect differences in the experimental approach. Phosphorylation at Thr-260 seems to play a role in the regulation of insulin secretion. Polyubiquitinated by the APC complex in conjunction with FZR1, leading to its proteasomal degradation. Targeted for proteasomal degradation by interaction with COPS5. BRSK2 levels change during the cell cycle. BRSK2 levels are low at the G1/S boundary and gradually increase as cells progress into G2 phase. BRSK2 levels decrease rapidly at the end of mitosis.
Function Serine/threonine-protein kinase that plays a key role in polarization of neurons and axonogenesis, cell cycle progress and insulin secretion. Phosphorylates CDK16, CDC25C, MAPT/TAU, PAK1 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in postmitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. Plays a role in the regulation of the mitotic cell cycle progress and the onset of mitosis. Plays a role in the regulation of insulin secretion in response to elevated glucose levels, probably via phosphorylation of CDK16 and PAK1. While BRSK2 phosphorylated at Thr-174 can inhibit insulin secretion, BRSK2 phosphorylated at Thr-260 can promote insulin secretion. Regulates reorganization of the actin cytoskeleton. May play a role in the apoptotic response triggered by endoplasmic reticulum (ER) stress.
Protein Name Serine/Threonine-Protein Kinase Brsk2
Brain-Selective Kinase 2
Brain-Specific Serine/Threonine-Protein Kinase 2
Br Serine/Threonine-Protein Kinase 2
Serine/Threonine-Protein Kinase 29
Serine/Threonine-Protein Kinase Sad-A
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Perinuclear Region
Endoplasmic Reticulum
Detected At Centrosomes During Mitosis
Localizes To The Endoplasmic Reticulum In Response To Stress Caused By Tunicamycin
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Brsk2 antibody
Anti-Brain-Selective Kinase 2 antibody
Anti-Brain-Specific Serine/Threonine-Protein Kinase 2 antibody
Anti-Br Serine/Threonine-Protein Kinase 2 antibody
Anti-Serine/Threonine-Protein Kinase 29 antibody
Anti-Serine/Threonine-Protein Kinase Sad-A antibody
Anti-BRSK2 antibody
Anti-C11orf7 antibody
Anti-PEN11B antibody
Anti-SADA antibody
Anti-STK29 antibody
Anti-HUSSY-12 antibody

Information sourced from Uniprot.org

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