Anti-Brk antibody (400-450) (STJ502711)

SKU:
STJ502711-100

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Host: Rabbit
Applications: ELISA/IHC/IP/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Brk (400-450) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Purification: Affinity Purified
Concentration: 0.5-1.5 µg/µl
Dilution Range: WB: 1:2, 500-1:5, 000
ELISA: 1:50, 000
IP: 1:200
Storage Instruction: Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Gene Symbol: Ptk6
Gene ID: 20459
Uniprot ID: PTK6_MOUSE
Immunogen Region: 400-450
Immunogen: Synthetic peptide taken within amino acid region 400-450 on mouse Protein-Tyrosine Kinase 6 isoform.
Post Translational Modifications Autophosphorylated. Autophosphorylation of Tyr-342 leads to an increase of kinase activity. Tyr-447 binds to the SH2 domain when phosphorylated and negatively regulates kinase activity.
Function Non-receptor tyrosine-protein kinase implicated in the regulation of a variety of signaling pathways that control the differentiation and maintenance of normal epithelia, as well as tumor growth. Function seems to be context dependent and differ depending on cell type, as well as its intracellular localization. A number of potential nuclear and cytoplasmic substrates have been identified. These include the RNA-binding proteins: KHDRBS1/SAM68, KHDRBS2/SLM1, KHDRBS3/SLM2 and SFPQ/PSF.transcription factors: STAT3 and STAT5A/B and a variety of signaling molecules: ARHGAP35/p190RhoGAP, PXN/paxillin, BTK/ATK, STAP2/BKS. Associates also with a variety of proteins that are likely upstream of PTK6 in various signaling pathways, or for which PTK6 may play an adapter-like role. These proteins include ADAM15, EGFR, ERBB2, ERBB3 and IRS4. In normal or non-tumorigenic tissues, PTK6 promotes cellular differentiation and apoptosis. In tumors PTK6 contributes to cancer progression by sensitizing cells to mitogenic signals and enhancing proliferation, anchorage-independent survival and migration/invasion. Association with EGFR, ERBB2, ERBB3 may contribute to mammary tumor development and growth through enhancement of EGF-induced signaling via BTK/AKT and PI3 kinase. Contributes to migration and proliferation by contributing to EGF-mediated phosphorylation of ARHGAP35/p190RhoGAP, which promotes association with RASA1/p120RasGAP, inactivating RhoA while activating RAS. EGF stimulation resulted in phosphorylation of PNX/Paxillin by PTK6 and activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. PTK6 activates STAT3 and STAT5B to promote proliferation. Nuclear PTK6 may be important for regulating growth in normal epithelia, while cytoplasmic PTK6 might activate oncogenic signaling pathways.
Protein Name Protein-Tyrosine Kinase 6
Src-Related Intestinal Kinase
Database Links Reactome: R-MMU-187577
Reactome: -MMU-69231
Reactome: -MMU-8847993
Reactome: -MMU-8849468
Reactome: -MMU-8849469
Reactome: -MMU-8849470
Reactome: -MMU-8849471
Reactome: -MMU-8849472
Reactome: -MMU-8849474
Reactome: -MMU-8857538
Cellular Localisation Cytoplasm
Nucleus
Membrane
Cell Projection
Ruffle
Also Found To Be Membrane-Associated
Colocalizes With Khdrbs1
Within The Nucleus
Alternative Antibody Names Anti-Protein-Tyrosine Kinase 6 antibody
Anti-Src-Related Intestinal Kinase antibody
Anti-Ptk6 antibody
Anti-Sik antibody

Information sourced from Uniprot.org

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