| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-BRIP1 (1-260) is suitable for use in Western Blot research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | BRIP1 |
| Gene ID: | 83990 |
| Uniprot ID: | FANCJ_HUMAN |
| Immunogen Region: | 1-260 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human BRIP1 (NP_114432.2). |
| Immunogen Sequence: | MSSMWSEYTIGGVKIYFPYK AYPSQLAMMNSILRGLNSKQ HCLLESPTGSGKSLALLCSA LAWQQSLSGKPADEGVSEKA EVQLSCCCACHSKDFTNNDM NQGTSRHFNYPSTPPSERNG TSSTCQDSPEKTTLAAKLSA KKQASIYRDENDDFQVEKKR IRPLETTQQIRKRHCFGTEV HNLDAKVDSGKTVKLNSPLE KINSFSPQKPPGHCSRCCCS TKQGNSQESSNTIKKDHTG |
| Tissue Specificity | Ubiquitously expressed, with highest levels in testis. |
| Post Translational Modifications | Phosphorylated. Phosphorylation is necessary for interaction with BRCA1, and is cell-cycle regulated. Acetylation at Lys-1249 facilitates DNA end processing required for repair and checkpoint signaling. |
| Function | DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1. |
| Protein Name | Fanconi Anemia Group J ProteinProtein FacjAtp-Dependent Rna Helicase Brip1Brca1-Associated C-Terminal Helicase 1Brca1-Interacting Protein C-Terminal Helicase 1Brca1-Interacting Protein 1 |
| Database Links | Reactome: R-HSA-2564830Reactome: R-HSA-5685938Reactome: R-HSA-5685942Reactome: R-HSA-5693554Reactome: R-HSA-5693568Reactome: R-HSA-5693579Reactome: R-HSA-5693607Reactome: R-HSA-5693616Reactome: R-HSA-6804756Reactome: R-HSA-69473Reactome: R-HSA-9701192Reactome: R-HSA-9704331Reactome: R-HSA-9704646Reactome: R-HSA-9709570Reactome: R-HSA-9709603 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Fanconi Anemia Group J Protein antibodyAnti-Protein Facj antibodyAnti-Atp-Dependent Rna Helicase Brip1 antibodyAnti-Brca1-Associated C-Terminal Helicase 1 antibodyAnti-Brca1-Interacting Protein C-Terminal Helicase 1 antibodyAnti-Brca1-Interacting Protein 1 antibodyAnti-BRIP1 antibodyAnti-BACH1 antibodyAnti-FANCJ antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89724/358205/STJ11100788_1__51624.1713122526.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")