| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
BRIP1 |
| Gene ID: |
83990 |
| Uniprot ID: |
FANCJ_HUMAN |
| Immunogen Region: |
1-260 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human BRIP1 (NP_114432.2). |
| Immunogen Sequence: |
MSSMWSEYTIGGVKIYFPYK AYPSQLAMMNSILRGLNSKQ HCLLESPTGSGKSLALLCSA LAWQQSLSGKPADEGVSEKA EVQLSCCCACHSKDFTNNDM NQGTSRHFNYPSTPPSERNG TSSTCQDSPEKTTLAAKLSA KKQASIYRDENDDFQVEKKR IRPLETTQQIRKRHCFGTEV HNLDAKVDSGKTVKLNSPLE KINSFSPQKPPGHCSRCCCS TKQGNSQESSNTIKKDHTG |
| Tissue Specificity | Ubiquitously expressed, with highest levels in testis. |
| Post Translational Modifications | Phosphorylated. Phosphorylation is necessary for interaction with BRCA1, and is cell-cycle regulated. Acetylation at Lys-1249 facilitates DNA end processing required for repair and checkpoint signaling. |
| Function | DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1. Involved in the repair of abasic sites at replication forks by promoting the degradation of DNA-protein cross-links: acts by catalyzing unfolding of HMCES DNA-protein cross-link via its helicase activity, exposing the underlying DNA and enabling cleavage of the DNA-protein adduct by the SPRTN metalloprotease. Can unwind RNA:DNA substrates. Unwinds G-quadruplex DNA.unwinding requires a 5'-single stranded tail. |
| Protein Name | Fanconi Anemia Group J ProteinBrca1-Associated C-Terminal Helicase 1Brca1-Interacting Protein C-Terminal Helicase 1Brca1-Interacting Protein 1Dna 5'-3' Helicase Fancj |
| Database Links | Reactome: R-HSA-2564830Reactome: R-HSA-5685938Reactome: R-HSA-5685942Reactome: R-HSA-5693554Reactome: R-HSA-5693568Reactome: R-HSA-5693579Reactome: R-HSA-5693607Reactome: R-HSA-5693616Reactome: R-HSA-6804756Reactome: R-HSA-69473Reactome: R-HSA-9701192Reactome: R-HSA-9704331Reactome: R-HSA-9704646Reactome: R-HSA-9709570Reactome: R-HSA-9709603 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Fanconi Anemia Group J Protein antibodyAnti-Brca1-Associated C-Terminal Helicase 1 antibodyAnti-Brca1-Interacting Protein C-Terminal Helicase 1 antibodyAnti-Brca1-Interacting Protein 1 antibodyAnti-Dna 5'-3' Helicase Fancj antibodyAnti-BRIP1 antibodyAnti-BACH1 antibodyAnti-FANCJ antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89724/358205/STJ11100788_1__51624.1713122526.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")