| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | BRIP1 |
| Gene ID: | 83990 |
| Uniprot ID: | FANCJ_HUMAN |
| Immunogen Region: | 1-260 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human BRIP1 (NP_114432.2). |
| Immunogen Sequence: | MSSMWSEYTIGGVKIYFPYK AYPSQLAMMNSILRGLNSKQ HCLLESPTGSGKSLALLCSA LAWQQSLSGKPADEGVSEKA EVQLSCCCACHSKDFTNNDM NQGTSRHFNYPSTPPSERNG TSSTCQDSPEKTTLAAKLSA KKQASIYRDENDDFQVEKKR IRPLETTQQIRKRHCFGTEV HNLDAKVDSGKTVKLNSPLE KINSFSPQKPPGHCSRCCCS TKQGNSQESSNTIKKDHTG |
| Tissue Specificity | Ubiquitously expressed, with highest levels in testis. |
| Post Translational Modifications | Phosphorylated. Phosphorylation is necessary for interaction with BRCA1, and is cell-cycle regulated. Acetylation at Lys-1249 facilitates DNA end processing required for repair and checkpoint signaling. |
| Function | DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1. Involved in the repair of abasic sites at replication forks by promoting the degradation of DNA-protein cross-links: acts by catalyzing unfolding of HMCES DNA-protein cross-link via its helicase activity, exposing the underlying DNA and enabling cleavage of the DNA-protein adduct by the SPRTN metalloprotease. Can unwind RNA:DNA substrates. Unwinds G-quadruplex DNA.unwinding requires a 5'-single stranded tail. |
| Protein Name | Fanconi Anemia Group J ProteinBrca1-Associated C-Terminal Helicase 1Brca1-Interacting Protein C-Terminal Helicase 1Brca1-Interacting Protein 1Dna 5'-3' Helicase Fancj |
| Database Links | Reactome: R-HSA-2564830Reactome: R-HSA-5685938Reactome: R-HSA-5685942Reactome: R-HSA-5693554Reactome: R-HSA-5693568Reactome: R-HSA-5693579Reactome: R-HSA-5693607Reactome: R-HSA-5693616Reactome: R-HSA-6804756Reactome: R-HSA-69473Reactome: R-HSA-9701192Reactome: R-HSA-9704331Reactome: R-HSA-9704646Reactome: R-HSA-9709570Reactome: R-HSA-9709603 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Fanconi Anemia Group J Protein antibodyAnti-Brca1-Associated C-Terminal Helicase 1 antibodyAnti-Brca1-Interacting Protein C-Terminal Helicase 1 antibodyAnti-Brca1-Interacting Protein 1 antibodyAnti-Dna 5'-3' Helicase Fancj antibodyAnti-BRIP1 antibodyAnti-BACH1 antibodyAnti-FANCJ antibody |
Information sourced from Uniprot.org
![Chromatin immunoprecipitation analysis of extracts of HeLa cells, using [KD Validated] CTCF Rabbit polyclonal antibody (STJ11100563) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89614/358098/STJ11100563_1__34552.1713122383.jpg?c=1 "Chromatin immunoprecipitation analysis of extracts of HeLa cells, using [KD Validated] CTCF Rabbit polyclonal antibody (STJ11100563) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.")
