• Immunofluorescence analysis of L929 cells using BRINP3 Rabbit polyclonal antibody (STJ11100194) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using BRINP3 Rabbit polyclonal antibody (STJ11100194) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-BRINP3 antibody (240-500) (STJ11100194)

SKU:
STJ11100194

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BRINP3 (240-500) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BRINP3
Gene ID: 339479
Uniprot ID: BRNP3_HUMAN
Immunogen Region: 240-500
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 240-500 of human BRINP3 (NP_950252.1).
Immunogen Sequence: LQGLQVLLPDYLQERFVQAA LSYIACNSEGEFICKENDCW CHCGPKFPECNCPSMDIQAM EENLLRITETWKAYNSDFEE SDEFKLFMKRLPMNYFLNTS TIMHLWTMDSNFQRRYEQLE NSMKQLFLKAQKIVHKLFSL SKRCHKQPLISLPRQRTSTY WLTRIQSFLYCNENGLLGSF SEETHSCTCPNDQVVCTAFL PCTVGDASACLTCAPDNRTR CGTCNTGYMLSQGLCKPEV
Tissue Specificity Strongly expressed in oral keratinocytes compared to the weak expression in tongue squamous cell carcinoma (SCC). Expressed in endothelial and aortic smooth muscle cells. Overexpressed in gonadotropinomas compared to normal pituitarie tissues.
Function Inhibits neuronal cell proliferation by negative regulation of the cell cycle transition. Promotes pituitary gonadotrope cell proliferation, migration and invasion, when overexpressed. May play a role in cell pituitary tumor development.
Protein Name Bmp/Retinoic Acid-Inducible Neural-Specific Protein 3
Dbccr1-Like Protein 1
Cellular Localisation Secreted
Mitochondrion
Alternative Antibody Names Anti-Bmp/Retinoic Acid-Inducible Neural-Specific Protein 3 antibody
Anti-Dbccr1-Like Protein 1 antibody
Anti-BRINP3 antibody
Anti-DBCCR1L antibody
Anti-DBCCR1L1 antibody
Anti-FAM5C antibody

Information sourced from Uniprot.org

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