Anti-BRAP antibody [1E7-C9-D10-B10] (STJ99215)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenPurified recombinant human BRAP protein fragments expressed in E.coli.
General Information
| Short Description | Mouse monoclonal anti-BRCA1-associated protein for use in WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 1E7-C9-D10-B10 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:1000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BRAP |
| Gene ID | 8315 |
| Uniprot ID | BRAP_HUMAN |
| Immunogen | Purified recombinant human BRAP protein fragments expressed in E.coli. |
| Specificity | This antibody detects endogenous levels of BRAP and does not cross-react with related proteins. |
Additional Info
| Function | Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport. |
| Protein Name | Brca1-Associated ProteinBrap2Impedes Mitogenic Signal PropagationImpRing Finger Protein 52Ring-Type E3 Ubiquitin Transferase Brap2Renal Carcinoma Antigen Ny-Ren-63 |
| Database Links | Reactome: R-HSA-5673000Reactome: R-HSA-5675221Reactome: R-HSA-6802946Reactome: R-HSA-6802955Reactome: R-HSA-9649948 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Brca1-Associated Protein antibodyAnti-Brap2 antibodyAnti-Impedes Mitogenic Signal Propagation antibodyAnti-Imp antibodyAnti-Ring Finger Protein 52 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Brap2 antibodyAnti-Renal Carcinoma Antigen Ny-Ren-63 antibodyAnti-BRAP antibodyAnti-RNF52 antibody |
Information sourced from Uniprot.org