Anti-BLK antibody (N-Term) (STJ72502)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-BLK (N-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 50kDa band observed in Human Thymus, Spleen and Lymph Node lysates (calculated MW of 57.7kDa according to NP_001706.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubat |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | BLK |
| Gene ID | 640 |
| Uniprot ID | BLK_HUMAN |
| Accession Number | NP_001706.2 |
| Immunogen Region | N-Term |
| Immunogen Sequence | QWSPLKVSAQDKD |
Additional Info
| Post Translational Modifications | Phosphorylated on tyrosine residues after antibody-mediated surface engagement of the B-cell antigen receptor (BCR). Ubiquitination of activated BLK by the UBE3A ubiquitin protein ligase leads to its degradation by the ubiquitin-proteasome pathway. |
| Function | Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor. Specifically binds and phosphorylates CD79A at 'Tyr-188'and 'Tyr-199', as well as CD79B at 'Tyr-196' and 'Tyr-207'. Phosphorylates also the immunoglobulin G receptors FCGR2A, FCGR2B and FCGR2C. With FYN and LYN, plays an essential role in pre-B-cell receptor (pre-BCR)-mediated NF-kappa-B activation. Contributes also to BTK activation by indirectly stimulating BTK intramolecular autophosphorylation. In pancreatic islets, acts as a modulator of beta-cells function through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose. Phosphorylates CGAS, promoting retention of CGAS in the cytosol. |
| Protein Name | Tyrosine-Protein Kinase BlkB Lymphocyte KinaseP55-Blk |
| Database Links | Reactome: R-HSA-8939245Reactome: R-HSA-983695 |
| Cellular Localisation | Cell MembraneLipid-AnchorPresent And Active In Lipid RaftsMembrane Location Is Required For The Phosphorylation Of Cd79a And Cd79b |
| Alternative Antibody Names | Anti-Tyrosine-Protein Kinase Blk antibodyAnti-B Lymphocyte Kinase antibodyAnti-P55-Blk antibodyAnti-BLK antibody |
Information sourced from Uniprot.org