Anti-Beta-nerve growth factor antibody (Native) (STJA0041016)
SPECIFICATIONS
ClonalityPolyclonal
HostSheep
ConjugationUnconjugated
IsotypeIgG
ImmunogenNative mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
General Information
| Short Description | Sheep polyclonal anti-Beta-nerve growth factor (Native) for use in ELISA, IHC-F, Neut and WB in Avian, Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/IHC-F/Neut/WB |
| Host | Sheep |
| Reactivity | Avian/Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity-purified |
| Dilution Range | WB 1-3 ยตg/mLIHC 1-3 ยตg/mL |
| Formulation | Lyophilized from a solution containing 0.1% Trehalose in PBS pH 7.4 |
| Storage Instruction | Spin vial briefly before opening. Reconstitute in 50 ยตL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at-20ยฐC for a higher stability, and at 2-8ยฐC with an appropriate antibacterial |
Target Information
| Gene Symbol | Ngf |
| Gene ID | 18049 |
| Uniprot ID | NGF_MOUSE |
| Immunogen | Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE) |
| Immunogen Region | Native |
| Immunogen Sequence | Mouse |
| Specificity | A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF. |
Additional Info
| Tissue Specificity | Detected in submaxillary gland (at protein level). Highly expressed in male submaxillary gland. Levels are much lower in female submaxillary gland. |
| Function | Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival. The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI. Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form. |
| Protein Name | Beta-Nerve Growth FactorBeta-Ngf |
| Database Links | Reactome: R-MMU-167060Reactome: -MMU-170968Reactome: -MMU-170984Reactome: -MMU-177504Reactome: -MMU-187042Reactome: -MMU-198203Reactome: -MMU-205017Reactome: -MMU-205025Reactome: -MMU-205043Reactome: -MMU-209543Reactome: -MMU-209560Reactome: -MMU-209563 |
| Cellular Localisation | SecretedEndosome LumenProngf Is Endocytosed After Binding To The Cell Surface Receptor Formed By Sort1 And Ngfr |
| Alternative Antibody Names | Anti-Beta-Nerve Growth Factor antibodyAnti-Beta-Ngf antibodyAnti-Ngf antibodyAnti-Ngfb antibody |
Information sourced from Uniprot.org