• Western blot analysis of Hela (1) Rat brain (2) Mouse brain (3) , Chicken lung (4) and Rabbit testis (5), diluted at 1:10000.
  • IHC Staining of Human ovary tissue, diluted at 1:200
  • Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, Beta-actin monoclonal antibody (5B7) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1, Beta-actin monoclonal antibody (5B7) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1, Beta-actin monoclonal antibody (5B7) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Western blot analysis of 1 HEK293 2 SW480 3 HEPG2 4 MCF-7 5 mouse brain 6 Rat brain 7 Hela 8 A549 lysates, primary antibody was diluted at 1:5000, 4°C over night, secondary antibody (cat: (NA was diluted at 1:10000, 37°C 1hour.
  • Immunofluorescence analysis of Hela cell. 1, Cyclin A Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-actin monoclonal antibody (5B7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Beta-actin antibody (STJ96930)

SKU:
STJ96930
Current Stock:
Host: Mouse
Applications: IF, WB, IHC-P
Reactivity: Human,Rat,Mouse,Monkey,Dog,Chimpanzee,Hamster,Rat,Rabbit,Insect
Short Description: Mouse monoclonal primary antibody against Beta-actin.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Monoclonal
Clone ID: 5B7
Conjugation: Unconjugated
Formulation: PBS, pH 7.4, containing 0.5% BSA, 0.02% sodium azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using a specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG1
Dilution Range: IF 1:50-200
WB 1:5000-20000
IHC 1:200-300
Specificity: The antibody detects endogenous beta-actin protein.
Uniprot ID: ACTB_HUMAN
Gene ID: 60
Gene Symbol: ACTB
Immunogen: Synthetic Peptide
Immunogen Region: C-term
Database Links Reactome: R-HSA-1445148
Cellular Localisation Cytoplasm
Cytoskeleton
Nucleus
Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
Protein Name Actin - Cytoplasmic 1
Beta-Actin Cleaved Into - Actin - Cytoplasmic 1 - N-Terminally Processed
Alternative Names Anti-Actin - Cytoplasmic 1 antibody
Anti-Beta-Actin Cleaved Into - Actin - Cytoplasmic 1 - N-Terminally Processed antibody
Anti-ACTB antibody

Information sourced from uniprot.org

6 months standard