• Western blot analysis of extracts of SH-SY5Y cells, using BCLAF1 antibody (STJ118901) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-BCLAF1 antibody (330-552) (STJ118901)

SKU:
STJ118901

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BCLAF1 (330-552) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BCLAF1
Gene ID: 9774
Uniprot ID: BCLF1_HUMAN
Immunogen Region: 330-552
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 330-552 of human BCLAF1 (NP_001070909.1).
Immunogen Sequence: TAKTGKFLKSPPLHKNLDAR EKSTFREESPLRIKMIASDS HRPEVKLKMAPVPLDDSNRP ASLTKDRLLASTLVHSVKKE QEFRSIFDHIKLPQASKSTS ESFIQHIVSLVHHVKEQYFK SAAMTLNERFTSYQKATEEH STRQKSPEIHRRIDISPSTL RKHTRLAGEERVFKEENQKG DKKLRCDSADLRHDIDRRRK ERSKERGDSKGSRESSGSRK QEK
Tissue Specificity Ubiquitous.
Post Translational Modifications Citrullinated by PADI4.
Function Death-promoting transcriptional repressor. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA.
Protein Name Bcl-2-Associated Transcription Factor 1
Btf
Bclaf1 And Thrap3 Family Member 1
Cellular Localisation Cytoplasm
Nucleus
Nucleus Speckle
Nucleoplasm
Alternative Antibody Names Anti-Bcl-2-Associated Transcription Factor 1 antibody
Anti-Btf antibody
Anti-Bclaf1 And Thrap3 Family Member 1 antibody
Anti-BCLAF1 antibody
Anti-BTF antibody
Anti-KIAA0164 antibody

Information sourced from Uniprot.org

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