• Western blot analysis of lysates from wild type (WT) and Bcl9 knockdown (KD) 293T cells using [KD Validated] Bcl9 Rabbit polyclonal antibody (STJ11105603) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of various lysates using [KD Validated] Bcl9 Rabbit polyclonal antibody (STJ11105603) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Bcl9 antibody (1278-1377) (STJ11105603)

SKU:
STJ11105603

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Bcl9 (1278-1377) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Bcl9
Gene ID: 77578
Uniprot ID: BCL9_MOUSE
Immunogen Region: 1278-1377
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1278-1377 of mouse Bcl9 (NP_084209.3).
Immunogen Sequence: DQAPRMGLALPGMGGPGPVG TPDIPLGTSPSMPGHNPMRP PAFLQQGMMGPHHRMMSPAQ STVPGPATLMTNPAAAVGMI PGKDRGPAGLYTHPGPVGSP
Function Promotes beta-catenin's transcriptional activity. Involved in signal transduction through the Wnt pathway.
Protein Name B-Cell Cll/Lymphoma 9 Protein
B-Cell Lymphoma 9 Protein
Bcl-9
Database Links Reactome: R-MMU-201722
Cellular Localisation Nucleus
Alternative Antibody Names Anti-B-Cell Cll/Lymphoma 9 Protein antibody
Anti-B-Cell Lymphoma 9 Protein antibody
Anti-Bcl-9 antibody
Anti-Bcl9 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance