![Western blot analysis of lysates from wild type (WT) and Bcl9 knockdown (KD) 293T cells using [KD Validated] Bcl9 Rabbit polyclonal antibody (STJ11105603) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178247/370554/STJ11105603_1__32101.1713136505.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Bcl9 knockdown (KD) 293T cells using [KD Validated] Bcl9 Rabbit polyclonal antibody (STJ11105603) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western blot analysis of various lysates using [KD Validated] Bcl9 Rabbit polyclonal antibody (STJ11105603) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178247/370555/STJ11105603_2__20243.1713136506.jpg?c=1 "Western blot analysis of various lysates using [KD Validated] Bcl9 Rabbit polyclonal antibody (STJ11105603) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western blot analysis of lysates from wild type (WT) and Bcl9 knockdown (KD) 293T cells using [KD Validated] Bcl9 Rabbit polyclonal antibody (STJ11105603) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/178247/370554/STJ11105603_1__32101.1713136505.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Bcl9 knockdown (KD) 293T cells using [KD Validated] Bcl9 Rabbit polyclonal antibody (STJ11105603) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | Bcl9 |
| Gene ID: | 77578 |
| Uniprot ID: | BCL9_MOUSE |
| Immunogen Region: | 1278-1377 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1278-1377 of mouse Bcl9 (NP_084209.3). |
| Immunogen Sequence: | DQAPRMGLALPGMGGPGPVG TPDIPLGTSPSMPGHNPMRP PAFLQQGMMGPHHRMMSPAQ STVPGPATLMTNPAAAVGMI PGKDRGPAGLYTHPGPVGSP |
| Function | Promotes beta-catenin's transcriptional activity. Involved in signal transduction through the Wnt pathway. |
| Protein Name | B-Cell Cll/Lymphoma 9 Protein B-Cell Lymphoma 9 Protein Bcl-9 |
| Database Links | Reactome: R-MMU-201722 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-B-Cell Cll/Lymphoma 9 Protein antibody Anti-B-Cell Lymphoma 9 Protein antibody Anti-Bcl-9 antibody Anti-Bcl9 antibody |
Information sourced from Uniprot.org