• Immunohistochemistry analysis of BCL2L14/BCLG in paraffin-embedded rat kidney tissue using BCL2L14/BCLG Rabbit monoclonal antibody (STJ11103020) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of BCL2L14/BCLG in paraffin-embedded Human lung adenocarcinoma tissue using BCL2L14/BCLG Rabbit monoclonal antibody (STJ11103020) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of BCL2L14/BCLG in paraffin-embedded rat testis tissue using BCL2L14/BCLG Rabbit monoclonal antibody (STJ11103020) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of BCL2L14/BCLG in paraffin-embedded mouse testis tissue using BCL2L14/BCLG Rabbit monoclonal antibody (STJ11103020) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of BCL2L14/BCLG in paraffin-embedded human colon tissue using BCL2L14/BCLG Rabbit monoclonal antibody (STJ11103020) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of BCL2L14/BCLG in paraffin-embedded mouse kidney tissue using BCL2L14/BCLG Rabbit monoclonal antibody (STJ11103020) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using BCL2L14/BCLG Rabbit monoclonal antibody (STJ11103020) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of various lysates using BCL2L14/BCLG Rabbit monoclonal antibody (STJ11103020) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-BCL2L14/BCLG antibody (1-250) [S0MR] (STJ11103020)

SKU:
STJ11103020

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-BCL2L14/BCLG (1-250) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BCL2L14
Gene ID: 79370
Uniprot ID: B2L14_HUMAN
Immunogen Region: 1-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human BCL2L14/BCLG (Q9BZR8).
Immunogen Sequence: MCSTSGCDLEEIPLDDDDLN TIEFKILAYYTRHHVFKSTP ALFSPKLLRTRSLSQRGLGN CSANESWTEVSWPCRNSQSS EKAINLGKKKSSWKAFFGVV EKEDSQSTPAKVSAQGQRTL EYQDSHSQQWSRCLSNVEQC LEHEAVDPKVISIANRVAEI VYSWPPPQATQAGGFKSKEI FVTEGLSFQLQGHVPVASSS KKDEEEQILAKIVELLKYSG DQLERKLKKDKALMGHFQD
Tissue Specificity Isoform 1 is widely expressed. Isoform 2 is testis-specific.
Post Translational Modifications Phosphorylated by MELK, leading to inhibit its pro-apoptotic function.
Function Plays a role in apoptosis.
Protein Name Apoptosis Facilitator Bcl-2-Like Protein 14
Bcl2-L-14
Apoptosis Regulator Bcl-G
Database Links Reactome: R-HSA-6803205
Cellular Localisation Cytoplasm
Isoform 1: Cytoplasm
Cytosol
Diffusely Distributed Throughout The Cytosol
Isoform 2: Endomembrane System
Predominantly Localized To Cytosolic Organelles
Alternative Antibody Names Anti-Apoptosis Facilitator Bcl-2-Like Protein 14 antibody
Anti-Bcl2-L-14 antibody
Anti-Apoptosis Regulator Bcl-G antibody
Anti-BCL2L14 antibody
Anti-BCLG antibody

Information sourced from Uniprot.org

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