Anti-BCKDK antibody [R01-3G4] (STJA0035127)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human BCKDK
General Information
| Short Description | Rabbit monoclonal anti-BCKDK for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R01-3G4 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | BCKDK |
| Gene ID | 10295 |
| Uniprot ID | BCKD_HUMAN |
| Immunogen | A synthetic peptide of human BCKDK |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Autophosphorylated. |
| Function | Serine/threonine-protein kinase component of macronutrients metabolism. Forms a functional kinase and phosphatase pair with PPM1K, serving as a metabolic regulatory node that coordinates branched-chain amino acids (BCAAs) with glucose and lipid metabolism via two distinct phosphoprotein targets: mitochondrial BCKDHA subunit of the branched-chain alpha-ketoacid dehydrogenase (BCKDH) complex and cytosolic ACLY, a lipogenic enzyme of Krebs cycle. Phosphorylates and inactivates mitochondrial BCKDH complex a multisubunit complex consisting of three multimeric components each involved in different steps of BCAA catabolism: E1 composed of BCKDHA and BCKDHB, E2 core composed of DBT monomers, and E3 composed of DLD monomers. Associates with the E2 component of BCKDH complex and phosphorylates BCKDHA on Ser-337, leading to conformational changes that interrupt substrate channeling between E1 and E2 and inactivates the BCKDH complex. Phosphorylates ACLY on Ser-455 in response to changes in cellular carbohydrate abundance such as occurs during fasting to feeding metabolic transition. Refeeding stimulates MLXIPL/ChREBP transcription factor, leading to increased BCKDK to PPM1K expression ratio, phosphorylation and activation of ACLY that ultimately results in the generation of malonyl-CoA and oxaloacetate immediate substrates of de novo lipogenesis and glucogenesis, respectively. Recognizes phosphosites having SxxE/D canonical motif. |
| Protein Name | Branched-Chain Alpha-Ketoacid Dehydrogenase KinaseBckdh KinaseBckdhkinBdk3-Methyl-2-Oxobutanoate Dehydrogenase Lipoamide Kinase - Mitochondrial |
| Database Links | Reactome: R-HSA-70895Reactome: R-HSA-9912481 |
| Cellular Localisation | Mitochondrion MatrixDetected In The Cytosolic Compartment Of Liver Cells |
| Alternative Antibody Names | Anti-Branched-Chain Alpha-Ketoacid Dehydrogenase Kinase antibodyAnti-Bckdh Kinase antibodyAnti-Bckdhkin antibodyAnti-Bdk antibodyAnti-3-Methyl-2-Oxobutanoate Dehydrogenase Lipoamide Kinase - Mitochondrial antibodyAnti-BCKDK antibody |
Information sourced from Uniprot.org